Ex vivo Dynamics of Human Glioblastoma Cells in a Microvasculature‐on‐a‐Chip System Correlates with Tumor Heterogeneity and Subtypes. Issue 8 (10th February 2019)

• Record Type: Journal Article
• Authors: Xiao, Yang; Kim, Dongjoo; Dura, Burak; Zhang, Kerou; Yan, Runchen; Li, Huamin; Han, Edward; Ip, Joshua; Zou, Pan; Liu, Jun; Chen, Ann Tai; Vortmeyer, Alexander O.; Zhou, Jiangbing; Fan, Rong
• Is Part Of: Advanced science. Volume 6:Issue 8(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-02-10
• Subjects: brain tumor dynamics -- ex vivo assays -- microvasculature -- organ‐on‐a‐chip; Science -- Periodicals; 505
• DOI: 10.1002/advs.201801531
• Languages: English
• ISSNs: 2198-3844

Abstract

Abstract: The perivascular niche (PVN) plays an essential role in brain tumor stem‐like cell (BTSC) fate control, tumor invasion, and therapeutic resistance. Here, a microvasculature‐on‐a‐chip system as a PVN model is used to evaluate the ex vivo dynamics of BTSCs from ten glioblastoma patients. BTSCs are found to preferentially localize in the perivascular zone, where they exhibit either the lowest motility, as in quiescent cells, or the highest motility, as in the invasive phenotype, with migration over long distance. These results indicate that PVN is a niche for BTSCs, while the microvascular tracks may serve as a path for tumor cell migration. The degree of colocalization between tumor cells and microvessels varies significantly across patients. To validate these results, single‐cell transcriptome sequencing (10 patients and 21 750 single cells in total) is performed to identify tumor cell subtypes. The colocalization coefficient is found to positively correlate with proneural (stem‐like) or mesenchymal (invasive) but not classical (proliferative) tumor cells. Furthermore, a gene signature profile including PDGFRA correlates strongly with the "homing" of tumor cells to the PVN. These findings demonstrate that the model can recapitulate in vivo tumor cell dynamics and heterogeneity, representing a new route to study patient‐specific tumor cell functions. Abstract : Incorporation of brain tumor cells from patients in a microvasculature‐on‐a‐chip system allows for quantifying localization and migration of individual tumor cells ex vivo. Comparing the results to single‐cell transcriptome sequencing reveals a correlation between ex vivo tumor cell dynamics and transcriptional heterogeneity.