A novel long non‐coding RNA regulates the immune response in MAC‐T cells and contributes to bovine mastitis. (12th March 2019)

• Record Type: Journal Article
• Authors: Wang, Hao; Wang, Xixi; Li, Xueru; Wang, Qianwen; Qing, Suzhu; Zhang, Yong; Gao, Ming‐Qing
• Is Part Of: FEBS journal. Volume 286:Number 9(2019)
• Page Start: 1780
• Page End: 1795
• Publication Date: 2019-03-12
• Subjects: bovine mammary epithelial cells -- lncRNA -- lncRNA‐TUB -- mastitis -- RNA sequence; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pathology, Molecular -- Periodicals; 572
• DOI: 10.1111/febs.14783
• Languages: English
• ISSNs: 1742-464X

Abstract

Abstract : The long non‐coding RNAs (lncRNAs) are known to transcriptionally regulate a wide spectrum of diseases. Here, we screened for potentially functional lncRNAs in a mammary epithelial cell model of bovine mastitis by RNA‐Seq technology and identified a class of previously undetected mastitis‐related lncRNAs. A novel lncRNA was widely expressed in a variety of bovine tissues with diverse relative abundance and had a relatively low expression in mammary tissue. Given its predicted target gene is TUBA1C, we name it lncRNA‐TUB . We found a higher expression of lncRNA‐TUB in mammary epithelial cells that received a proinflammatory stimulus compared to normal cells. Knockout of lncRNA‐TUB by the CRISPR/Cas9 system revealed that it plays crucial roles in the morphological shape, proliferation, migration and β‐casein secretion of mammary epithelial cells. In addition, lncRNA‐TUB mediates Escherichia coli ‐induced inflammatory factor secretion and Staphylococcus aureus adhesion to epithelial cells. Our results suggest that the lncRNAs identified here function in bovine mastitis, and that lncRNA‐TUB affects the basic biological characteristics and functions of bovine mammary epithelial cells in inflammatory conditions, providing valuable insights into the mechanisms of bovine mastitis. Abstract : In this study, it is shown that loss of a long non‐coding RNA named lncRNA‐TUB causes a significant change in the expression of TUBA1C and TGF‐β1. Lower expression of TUBA1C may directly change the cytoskeleton of MAC‐T cells, whereas higher expression of TGF‐β1 promotes an epithelial–mesenchymal transition‐like morphology via the TGF‐β1–Smad pathway. More importantly, loss of lncRNA‐TUB leads to the abnormal immune response of MAC‐T cell.