Diurnal protein oscillation profiles in Drosophila head. Issue 22 (30th October 2018)

• Record Type: Journal Article
• Authors: Du, Juan; Zhang, Yifan; Xue, Yongbo; Zhao, Xiaoyun; Zhao, Xianguo; Wei, Yu; Li, Zhen; Zhang, Yong; Zhao, Zhangwu
• Is Part Of: FEBS letters. Volume 592:Issue 22(2018)
• Page Start: 3736
• Page End: 3749
• Publication Date: 2018-10-30
• Subjects: circadian rhythm -- clock -- Drosophila -- proteomics; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.13267
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Circadian clocks control daily rhythms in physiology, metabolism and behaviour in most organisms. Proteome‐wide analysis of protein oscillations is still lacking in Drosophila . In this study, the total protein and phosphorylated protein in Drosophila heads in a 24‐h daily time‐course were assayed by using the isobaric tags for relative and absolute quantitation (iTRAQ) method, and 10 and 7 oscillating proteins as well as 19 and 22 oscillating phosphoproteins in the w 1118 control and Clk Jrk mutant strains were separately identified. Lastly, we performed a mini screen to investigate the functions of some oscillating proteins in circadian locomotion rhythms. This study provides the first proteomic profiling of diurnally oscillating proteins in fly heads, thereby providing a basis for further mechanistic studies of these proteins in circadian rhythm. Abstract :