Codon harmonization – going beyond the speed limit for protein expression. Issue 9 (16th April 2018)

• Record Type: Journal Article
• Authors: Mignon, Charlotte; Mariano, Natacha; Stadthagen, Gustavo; Lugari, Adrien; Lagoutte, Priscillia; Donnat, Stéphanie; Chenavas, Sylvie; Perot, Cyril; Sodoyer, Régis; Werle, Bettina
• Is Part Of: FEBS letters. Volume 592:Issue 9(2018)
• Page Start: 1554
• Page End: 1564
• Publication Date: 2018-04-16
• Subjects: Codon Adaptation Index -- Escherichia coli -- heterologous protein expression -- yield improvement; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.13046
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Codon usage distribution has been soundly used by nature to fine tune protein biogenesis. Alteration of the mRNA structure or sequential scheduling of codons can profoundly affect translation, thus altering protein yield, functionality, solubility, and proper folding. Building on these observations, here, we present an evaluation of different recently designed algorithms of sequence adaptation based on Codon Adaptation Index (CAI) profiling. The first algorithm globally harmonizes synonymous codons in the original sequence in full respect to the heterologous expression host codon usage. The second recodes the sequence in accordance with the native sequence CAI profile. Our data, generated on three model proteins, highlights the importance to consider gene recoding as a parameter itself for recombinant protein expression improvement. Abstract :