EGb 761 inhibits Aβ1–42-induced neuroinflammatory response by suppressing P38 MAPK signaling pathway in BV-2 microglial cells. Issue 6 (10th April 2019)

• Record Type: Journal Article
• Authors: Meng, Miaomiao; AI, Di; Sun, Lingzhi; Xu, Xiangqing; Cao, Xiaolan
• Is Part Of: NeuroReport. Volume 30:Issue 6(2019)
• Publication Date: 2019-04-10
• Subjects: Alzheimer's disease -- EGb 761 -- neuroinflammatory response -- nuclear factor-κB -- P38 MAPK; Neurosciences -- Periodicals; Nervous system -- Periodicals; Neurophysiology -- Periodicals; Nervous System Diseases -- Periodicals; Nervous System Physiological Phenomena -- Periodicals; Neurosciences -- Periodicals; 616.805
• DOI: 10.1097/WNR.0000000000001223
• Languages: English
• ISSNs: 0959-4965

Abstract

Abstract : Ginkgo biloba extract EGb 761 possesses a variety of biological effects and has been proved to be beneficial in Alzheimer's disease. This study aimed to explore the anti-inflammatory mechanisms of EGb 761 on the Aβ1–42 -induced BV-2 microglial cells. We analyzed the production and gene expression of proinflammatory cytokines by enzyme-linked immunosorbent assay and qRT-PCR, examined phosphorylation of MAPKs by western blot and measured nuclear factor-κB nuclear translocation. Compared with Aβ1–42 -treated group, EGb 761 inhibited release and gene expression of tumor necrosis factor-α and interleukin-1β, suppressed nuclear translocation of nuclear factor-κB and attenuated phosphorylation of p38 MAPK in a concentration-dependent manner, but not ERK and JNK. In summary, the results suggested that EGb 761 could attenuate Aβ1–42 -induced neuroinflammatory response.