Mineralized alginate hydrogels using marine carbonates for bone tissue engineering applications. (1st September 2018)

• Record Type: Journal Article
• Authors: Diaz-Rodriguez, P.; Garcia-Triñanes, P.; Echezarreta López, M.M.; Santoveña, A.; Landin, M.
• Is Part Of: Carbohydrate polymers. Volume 195(2018)
• Page Start: 235
• Page End: 242
• Publication Date: 2018-09-01
• Subjects: Biominerals -- Mineralization -- Bone -- Hydrogel -- Marine carbonate particles -- Tissue engineering; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.04.101
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: CaCO3 mineralized alginate hydrogels promoted osteogenic differentiation of MSCs. Alginate composition modulates hydrogel mechanical properties and cell responses. CaCO3 particle characteristics depend on the source material selected for production. Abstract: The search for an ideal bone tissue replacement has led to the development of new composite materials designed to simulate the complex inorganic/organic structure of bone. The present work is focused on the development of mineralized calcium alginate hydrogels by the addition of marine derived calcium carbonate biomineral particles. Following a novel approach, we were able to obtain calcium carbonate particles of high purity and complex micro and nanostructure dependent on the source material. Three different types of alginates were selected to develop inorganic/organic scaffolds in order to correlate alginate composition with scaffold properties and cell behavior. The incorporation of calcium carbonates into alginate networks was able to promote extracellular matrix mineralization and osteoblastic differentiation of mesenchymal stem cells when added at 7 mg/ml. We demonstrated that the selection of the alginate type and calcium carbonate origin is crucial to obtain adequate systems for bone tissue engineering as they modulate the mechanical properties and cell differentiation.