Comparison of cell mechanical measurements provided by Atomic Force Microscopy (AFM) and Micropipette Aspiration (MPA). (July 2019)

• Record Type: Journal Article
• Authors: Daza, Rafael; González-Bermúdez, Blanca; Cruces, Julia; De la Fuente, Mónica; Plaza, Gustavo R.; Arroyo-Hernández, María; Elices, Manuel; Pérez-Rigueiro, José; Guinea, Gustavo V.
• Is Part Of: Journal of the mechanical behavior of biomedical materials. Volume 95(2019)
• Page Start: 103
• Page End: 115
• Publication Date: 2019-07
• Subjects: Mechanobiology -- T cell -- Elastic modulus -- Classical models -- Finite size effect; Biomedical materials -- Periodicals; Biomedical materials -- Mechanical properties -- Periodicals; Biomedical materials; Biomedical materials -- Mechanical properties; Periodicals; Electronic journals; 610.28
• DOI: 10.1016/j.jmbbm.2019.03.031
• Languages: English
• ISSNs: 1751-6161

Abstract

Abstract: A comparative analysis of T-lymphocyte mechanical data obtained from Micropipette Aspiration (MPA) and Atomic Force Microscopy (AFM) is presented. Results obtained by fitting the experimental data to simple Hertz and Theret models led to non-Gaussian distributions and significantly different values of the elastic moduli obtained by both techniques. The use of more refined models, taking into account the finite size of cells (simplified double contact and Zhou models) reduces the differences in the values calculated for the elastic moduli. Several possible sources for the discrepancy between the techniques are considered. The analysis suggests that the local nature of AFM measurements compared with the more general character of MPA measurements probably contributed to the differences observed.