Polyamines protect nucleic acids against depurination. (June 2018)

• Record Type: Journal Article
• Authors: Terui, Yusuke; Yoshida, Taketo; Sakamoto, Akihiko; Saito, Daisuke; Oshima, Tairo; Kawazoe, Masahito; Yokoyama, Shigeyuki; Igarashi, Kazuei; Kashiwagi, Keiko
• Is Part Of: International journal of biochemistry & cell biology. Volume 99(2018)
• Page Start: 147
• Page End: 153
• Publication Date: 2018-06
• Subjects: Polyamine -- Depurination -- Thermus thermophilus -- Standard polyamines -- Unusual polyamines; Biochemistry -- Periodicals; Cytology -- Periodicals; Biochemistry -- Periodicals; Cell Biology -- Periodicals; Biochimie -- Périodiques; Cytologie -- Périodiques; Biochimie; Cytologie; Biochemistry; Cytology; Ressource Internet (Descripteur de forme); Périodique électronique (Descripteur de forme); Periodicals; 572.05
• DOI: 10.1016/j.biocel.2018.04.008
• Languages: English
• ISSNs: 1357-2725

Abstract

Highlights: Depurination of nucleic acids was suppressed by standard and unusual polyamines. Standard polyamines suppressed depurination of RNA more effectively than that of DNA. Long linear polyamine, caldohexamine, most effectively suppressed depurination. Branched tetrakis(3-aminopropyl)ammonium suppressed depurination of RNA effectively. Abstract: Depurination is accelerated by heat and reactive oxygen species under physiological conditions. We previously reported that polyamines are involved in mitigation of heat shock and oxidative stresses through stimulation of the synthesis of heat shock and antioxidant proteins. This time, we investigated whether polyamines are directly involved in protecting nucleic acids from thermal depurination induced by high temperature. The suppressing efficiencies of depurination of DNA by spermine, caldopentamine and caldohexamine in the presence of 1 mM Mg 2+, were approximately 50%, 60% and 80%, respectively. Mg 2+ also protected nucleic acids against depurination but to a lesser degree than polyamines. Longer unusual polyamines were more effective at protecting DNA against depurination compared to standard polyamines. The tRNA depurination suppressing efficiencies of spermine, caldopentamine and caldohexamine in the presence of 1 mM Mg 2+, were approximately 60%, 70% and 80%, respectively. Standard polyamines protected tRNA and ribosomes more effectively than DNA against thermal depurination. Branched polyamines such as mitsubishine and tetrakis(3-aminopropyl)ammonium also protected RNA more effectively than DNA against depurination. These results suggest that the suppressing effect of depurination of nucleic acids (DNA and RNA) depends on the types of polyamines: i.e. to maintain functional conformation of nucleic acids at high temperature, longer and branched polyamines play important roles in protecting nucleic acids from depurination compared to standard polyamines and Mg 2+ .