Specific stabilization of DNA G-quadruplex structures with a chemically modified complementary probe. Issue 10 (15th May 2019)

• Record Type: Journal Article
• Authors: Liu, Min; Liu, Yaqian; Wu, Fan; Du, Yuhao; Zhou, Xiang
• Is Part Of: Bioorganic & medicinal chemistry. Volume 27:Issue 10(2019)
• Page Start: 1962
• Page End: 1965
• Publication Date: 2019-05-15
• Subjects: G-quadruplex -- In situ hybridization -- Gene repression -- Polymerase Stop Assay; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; Biochemistry -- Periodicals; Chemistry, Clinical -- Periodicals; Chemistry, Organic -- Periodicals; Chimie bio-organique -- Périodiques; Chimie pharmaceutique -- Périodiques; 615.19
• DOI: 10.1016/j.bmc.2019.03.053
• Languages: English
• ISSNs: 0968-0896

Abstract

Graphical abstract: Abstract: The DNA G-quadruplex is an important higher-order structure formed from guanine-rich DNA sequences. There are many molecules which can stabilize this structure. However, the selectivity of these ligands to different G-quadruplexes was not satisfactory. Herein, we designed and synthesized a chemically modified G-quadruplex probe, Razo-DNA, for the unique stabilization of the G-quadruplex. Razo-DNA consists of two fragments: The first is an organic molecular moiety which can stabilize G-quadruplex structures, and the second is a DNA molecule that is complementary with a sequence adjacent to the guanine-rich sequence of targeted DNA. Further studies showed that Razo-DNA could precisely stabilize the targeted DNA G-quadruplex structures in vitro.