Impact of conjugated linoleic acid administered to rats prior and after carcinogenic agent on arachidonic and linoleic acid metabolites in serum and tumors. (November 2017)

• Record Type: Journal Article
• Authors: Jelińska, Małgorzata; Białek, Agnieszka; Gielecińska, Iwona; Mojska, Hanna; Tokarz, Andrzej
• Is Part Of: Prostaglandins, leukotrienes, and essential fatty acids. Volume 126(2017)
• Page Start: 1
• Page End: 8
• Publication Date: 2017-11
• Subjects: AA arachidonic acid -- CLA conjugated linoleic acid -- COX cyclooxygenase -- EPA eicosapentaenoic acid -- DGLA dihomo-gamma-linolenoic acid -- DHA docosahexaenoic acid -- DMBA 7, 12-dimethylbenz[a]anthracene -- FLAP 5-lipoxygenase activating protein -- HETE hydroxyeicosatetraenoic acid -- HODE hydroxyoctadecadienoic acid -- LA linoleic acid -- LC-MS/MS liquid chromatography with tandem mass spectrometry -- LOX lipoxygenase -- 5-oxo-ETE 5-oxo-eicosatetraenoic acid -- PGE2 prostaglandin E2 -- PPARγ peroxisome proliferator-activated receptor gamma -- PUFA polyunsaturated fatty acids -- SEM standard error of mean -- TXA2 thromboxane A2; Hydroxyeicosatetraenoic acids -- 15- -- 12- and 5-HETE -- Hydroxyoctadecadienoic acids -- 13- and 9-HODE -- Prostaglandin E2 -- Conjugated linoleic acid; Lipids -- Periodicals; Unsaturated fatty acids -- Periodicals; Prostaglandins -- Periodicals; Leukotrienes -- Periodicals; Fatty Acids, Unsaturated -- Periodicals; Acides gras insaturés -- Périodiques; Prostaglandines -- Périodiques; Leucotriènes -- Périodiques; Lipides -- Périodiques; 612.01577
• DOI: 10.1016/j.plefa.2017.08.013
• Languages: English
• ISSNs: 0952-3278

Abstract

Abstract: The objective of the study was to assess the influence of conjugated linoleic acid (CLA) daily supplementation prior and after carcinogenic agent on the concentrations of eicosanoids – metabolites of arachidonic acid (15-, 12- or 5-hydroxyeicosatetraenoic acids (15-, 12-, 5-HETE), prostaglandin E2 (PGE2 )) and linoleic acid (13- or 9-hydroxyoctadecadienoic acids (13-, 9-HODE)) in rat serum and 7, 12-dimethylbenz[ a ]anthracene (DMBA)-induced tumors. Female rats were randomised into six groups, receiving 1% or 2% Bio-C.L.A or plant oil since the 37th day of life throughout the whole experiment. Some rats (50-day-old) were administered DMBA to induce tumors. Eicosanoids were analyzed with LC-MS/MS. The study indicated that CLA supplemented daily to rats prior and after carcinogen administration affected concentrations of arachidonic and linoleic acid metabolites in rat serum and induced tumors. However, ratios of eicosanoids exerting opposite activities (e.g. 12-HETE/15-HETE) appear to act as more precise factors reflecting pathological changes in an organism than individual compounds. Highlights: Effect of CLA on arachidonic and linoleic acid metabolites in rats was studied. The carcinogen-treated rats were supplemented daily with various doses of CLA. 15-HETE, 12-HETE, 5-HETE, 13-HODE, 9-HODE and PGE2 were analyzed. CLA affected linoleic and arachidonic acid metabolite levels in serum and tumors. Ratios of adverse effect metabolites are better indicators of pathological process.