Continuous exposure to α-glycosyl isoquercitrin from developmental stage facilitates fear extinction learning in rats. (April 2019)

• Record Type: Journal Article
• Authors: Okada, Rena; Masubuchi, Yasunori; Tanaka, Takaharu; Nakajima, Kota; Masuda, Sosuke; Nakamura, Kazuki; Maronpot, Robert R.; Yoshida, Toshinori; Koyanagi, Mihoko; Hayashi, Shim-mo; Shibutani, Makoto
• Is Part Of: Journal of functional foods. Volume 55(2019)
• Page Start: 312
• Page End: 324
• Publication Date: 2019-04
• Subjects: α-glycosyl isoquercitrin (AGIQ) -- Fear extinction -- Rat -- Hippocampus -- Post-traumatic stress disorder (PTSD) -- Synaptic plasticity; AGIQ α-glycosyl isoquercitrin -- ALA α-lipoic acid -- ARC activity-regulated cytoskeleton-associated protein -- BW body weight -- CALB2 calbindin-D–29K -- COX2 cyclooxygenase 2 -- CT threshold cycle -- DAB 3, 3′-diaminobenzidine -- DCX doublecortin -- FOS Fos proto-oncogene, AP-1 transcription factor subunit -- GABA γ-aminobutyric acid -- GCL granule cell layer -- GD gestational day -- GFAP glial fibrillary acidic protein -- IEG immediate-early gene -- LTP long-term potentiation -- NeuN neuronal nuclei -- NMDA N-methyl-d-aspartate -- NMDAR NMDA receptor -- PCNA proliferating cell nuclear antigen -- p-ERK1/2 phosphorylated extracellular signal-regulated kinase 1/2 (MAP kinase1/2) -- PFA paraformaldehyde -- PND postnatal day -- PTSD post-traumatic stress disorder -- PVALB parvalbumin -- RELN reelin -- ROS reactive oxygen species -- RT reverse transcription -- SGZ subgranular zone -- SOX2 SRY (sex determining region Y)-box 2 -- SST somatostatin -- TBR2 T-box brain protein 2 -- TUNEL terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2019.02.024
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: AGIQ enhanced fear extinction learning at the 3rd extinction trial. AGIQ upregulated neuronal plasticity-related gene products in brain regions. AGIQ upregulated Kif21b, encoding a memory-rewriting molecule in the hippocampus. AGIQ exposure may mitigate some stress disorders. Abstract: This study investigated the effect of exogenously administered polyphenolic antioxidant, α-glycosyl isoquercitrin (AGIQ), on neural function in rats following dietary exposure at 0.5% from gestational day 6 to postnatal day 77, in comparison with nonpolyphenolic α-lipoic acid (ALA) at 0.2%. AGIQ-exposed offspring revealed enhanced fear extinction learning at the 3rd trial and transcript upregulation of Fos and Kif21b in the hippocampal dentate gyrus and Grin2d in the amygdala. AGIQ also increased the number of FOS-immunoreactive (+) hippocampal granule cells. While ALA showed fear extinction learning-related transcript expression changes, it changed neither fear extinction learning nor FOS + cell numbers. The increases of FOS + cells and Grin2d transcripts by AGIQ suggested an association with increase of synaptic plasticity, leading to enhancement of fear extinction learning. AGIQ may also upregulate KIF21B, a recently identified memory-rewriting molecule. The present study findings suggest a possible therapeutic use of AGIQ in mitigating some stress disorders.