Guaraná (Paullinia cupana) catechins and procyanidins: Gastrointestinal/colonic bioaccessibility, Caco-2 cell permeability and the impact of macronutrients. (April 2019)

• Record Type: Journal Article
• Authors: Mendes, Thaíse Maria Nogueira; Murayama, Yukihisa; Yamaguchi, Natsumi; Sampaio, Geni Rodrigues; Fontes, Luciana Cristina Brigatto; Torres, Elizabeth Aparecida Ferraz da Silva; Tamura, Hirotoshi; Yonekura, Lina
• Is Part Of: Journal of functional foods. Volume 55(2019)
• Page Start: 352
• Page End: 361
• Publication Date: 2019-04
• Subjects: Caco-2 cells -- Catechin -- Guaraná -- In vitro digestion -- Macronutrients -- Casein; Papp apparent permeability; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2019.02.026
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Guaraná catechin monomers and dimers can permeate across Caco-2 cell monolayers. Intestinal permeation of guaraná catechins not changed by additional macronutrients. Oil improves the bioaccessibility of total procyanidins. A fast and sensitive isocratic HPLC-UV method was developed for guaraná catechins. Abstract: Guaraná ( Paullinia cupana ) seeds are rich in flavan-3-ols (catechins) and reduce oxidative stress in humans. We determined the concentration and in vitro bioaccessibility of catechins in guaraná seeds from two geographical origins, and measured their Caco-2 permeability. The effect of macronutrients on catechins' bioaccessibility and Caco-2 permeability was also evaluated. Guaraná seeds from southern Bahia and Maués contained, respectively, 30.0 and 26.8 mg catechin/g, 20.2 and 20.0 mg epicatechin/g, 3.7 and 3.8 mg procyanidin B1/g, and 3.3 and 4.9 mg procyanidin B2/g. Bioaccessibility ranged between 65 and 95% for catechin monomers and 50–140% for dimers. Casein inhibited bioaccessibility of catechin dimers, while vegetable oil improved bioaccessibility of total procyanidins. Guaraná catechin monomers and dimers were able to permeate through Caco-2 cell monolayers (Papp 0.31–0.35 × 10 −6 cm/s) without effect from co-digested macronutrients. Guaraná seeds are a source of highly bioaccessible catechins, which can permeate the intestinal epithelium with negligible effect from macronutrients.