Rapidly screening of α-glucosidase inhibitors from Dioscorea opposita Thunb. peel based on rGO@Fe3O4 nanocomposites microreactor. (1st January 2018)

• Record Type: Journal Article
• Authors: Zhang, Songsong; Qiu, Beibei; Zhu, Jinhua; Hu, Weiping; Ma, Fanyi; Khan, M. Z. H.; Liu, Xiuhua
• Is Part Of: Journal of enzyme inhibition and medicinal chemistry. Volume 33:Number 1(2018)
• Page Start: 1335
• Page End: 1342
• Publication Date: 2018-01-01
• Subjects: rGO@Fe3O4 nanocomposite -- enzymatic microreactors -- α-glucosidase inhibitors -- Chinese Yam (Dioscorea opposita Thunb.); Enzyme inhibitors -- Periodicals; Enzyme Inhibitors -- periodicals; Biochemistry -- periodicals; 572.7
• DOI: 10.1080/14756366.2018.1493472
• Languages: English
• ISSNs: 1475-6366

Abstract

Abstract: Present study aimed to immobilise the α-glucosidase on suitable supports to construct enzymatic microreactors and their subsequent applicability in efficient inhibitor screening from the Chinese Yam ( Dioscorea opposita Thunb.) peel. A type of lamellar and porous composites (rGO@Fe3 O4 ) were synthesised with a facile one-step solvothermal method and employed as carriers to construct enzymatic microreactors for screening α-glucosidase ligand from the Chinese Yam peel in league with the high performance liquid chromatography and mass spectrometry (HPLC-MS). The immobilisation amount of α-glucosidase on rGO@Fe3 O4 under the optimised conditions was about 40 μg α-glucosidase/mg carriers. Furthermore, the binding capacities of screened inhibitors, 2, 4-dimethoxy-6, 7-dihydroxyphenanthrene and batatasin I, were 35.6 and 68.2%, respectively. Hence, considering their high screening efficiency and excellent magnetic separation ability, these as-prepared nanocomposite consisting of rGO and Fe3 O4 may be potential supports for the enzyme (such as α-glucosidase) immobilisation for rapid α-glucosidase inhibitors screening from the diverse nature resources.