A Photoacoustic Probe for the Imaging of Tumor Apoptosis by Caspase‐Mediated Macrocyclization and Self‐Assembly. Issue 15 (20th February 2019)

• Record Type: Journal Article
• Authors: Wang, Yuqi; Hu, Xiaoming; Weng, Jianhui; Li, Jinbo; Fan, Quli; Zhang, Yan; Ye, Deju
• Is Part Of: Angewandte Chemie international edition. Volume 58:Issue 15(2019)
• Page Start: 4886
• Page End: 4890
• Publication Date: 2019-02-20
• Subjects: activatable probes -- apoptosis -- caspase-3 -- photoacoustic imaging -- self-assembly; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201813748
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Photoacoustic (PA) imaging shows promise in the sensitive detection of caspase‐3 activated in early tumor apoptosis in response to chemotherapy; smart PA probes are thus in high demand. Herein, we report the first smart PA probe (1‐RGD ) responsive to caspase‐3, enabling real‐time and high‐resolution imaging of tumor apoptosis.1‐RGD is designed to leverage the synergetic effect of active delivery and caspase‐3 activation. It is selectively recognized by active caspase‐3 to trigger peptide substrate cleavage and biocompatible macrocyclization‐mediated self‐assembly, leading to an amplified PA imaging signal and prolonged retention in apoptotic tumor cells. Strong, high‐resolution PA images are obtained in chemotherapy‐induced apoptotic tumors in living mice after intravenous administration with1‐RGD, facilitating sensitive reporting of caspase‐3 activity and distribution within tumor tissues. Abstract : Good vibrations : The caspase‐3‐mediated biocompatible macrocyclization and self‐assembly of a photoacoustic (PA) probe, 1‐RGD, into indocyanine green (ICG)‐containing nanoparticles is reported. The probe allows the enhancement of PA imaging signals for the real‐time detection of tumor apoptosis in living mice with high sensitivity and spatial resolution.