Cooperative binding of anti-tetanus toxin monoclonal antibodies: Implications for designing an efficient biclonal preparation to prevent tetanus toxin intoxication. Issue 26 (18th June 2018)

• Record Type: Journal Article
• Authors: Lukic, Ivana; Filipovic, Ana; Inic-Kanada, Aleksandra; Marinkovic, Emilija; Miljkovic, Radmila; Stojanovic, Marijana
• Is Part Of: Vaccine. Volume 36:Issue 26(2018)
• Page Start: 3764
• Page End: 3771
• Publication Date: 2018-06-18
• Subjects: Antibodies -- Cooperative effect -- Protection -- Tetanus toxin; Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2018.05.058
• Languages: English
• ISSNs: 0264-410X

Abstract

Graphical abstract: Abstract: Oligoclonal combinations of several monoclonal antibodies (MAbs) are being considered for the treatment of various infectious pathologies. These combinations are less sensitive to antigen structural changes than individual MAbs; at the same time, their characteristics can be more efficiently controlled than those of polyclonal antibodies. The main goal of this study was to evaluate the binding characteristics of six biclonal equimolar preparations (BEP) of tetanus toxin (TeNT)-specific MAbs and to investigate how the MAb combination influences the BEPs' protective capacity. We show that a combination of TeNT-specific MAbs, which not only bind TeNT but also exert positive cooperative effects, results in a BEP with superior binding characteristics and protective capacity, when compared with the individual component MAbs. Furthermore, we show that a MAb with only partial protective capacity but positive effects on the binding of the other BEP component can be used as a valuable constituent of the BEP.