Chemically crosslinked gelatin hydrogels as scaffolding materials for adipose tissue engineering. Issue 8 (3rd October 2018)

• Record Type: Journal Article
• Authors: Contessi Negrini, N.; Tarsini, P.; Tanzi, M. C.; Farè, S.
• Is Part Of: Journal of applied polymer science. Volume 136:Issue 8(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-10-03
• Subjects: 3T3‐L1 cells -- adipose tissue engineering -- crosslinked gelatin hydrogels -- mechanical properties -- rheological properties; Polymers -- Periodicals; Polymerization -- Periodicals; 668.9
• DOI: 10.1002/app.47104
• Languages: English
• ISSNs: 0021-8995

Abstract

ABSTRACT: The design of scaffolding materials that mimic the properties of the target tissue to be regenerated is a mandatory requirement to engineer a successful scaffold; however, the heterogeneous properties of adipose tissue (AT), strictly dependent on the AT depot, are often underestimated when engineering AT scaffolds. Moreover, a scaffolding material with versatile properties, suitable for the regeneration of different AT depots, is currently missing. Chemically crosslinked gelatin hydrogels are here prepared, and their properties tuned by varying gelatin concentration and reaction stoichiometry to obtain hydrogels suitable for AT regeneration. All hydrogel formulations are stable in water at 37 °C, showing swelling behavior dependent on synthesis parameters. The mechanical compressive response mimics the viscoelastic response typical of native AT, with elastic modulus values covering the range of breast and heel pad AT. The rheological properties vary among the hydrogel formulations, showing a typical shear thinning response, comparable to other AT scaffolds described in literature. In vitro cytotoxicity tests using 3T3‐L1 preadipocytes show no cytotoxic effects up to 7 days. 3T3‐L1 cells seeded on the hydrogels show good adhesion, proliferation, and adipogenic differentiation, confirmed by an increase in peroxisome proliferator‐activated receptor gamma gene expression and lipid droplets accumulation observed by Oil Red O staining. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci.2019, 136, 47104. Abstract : Regeneration of adipose tissue (AT) requires scaffolding materials with versatile properties, suitable to mimic the AT depot to be regenerated. Chemically crosslinked gelatin hydrogels, synthesized by varying gelatin concentration and reaction stoichiometry, show versatile and ideal physicomechanical properties for AT regenerations; the hydrogels sustain in vitro preadipocytes adhesion, proliferation, and adipogenic differentiation.