Imaging two targets in live cells based on rational design of lanthanide organic structure appended carbon dots. (November 2015)

• Record Type: Journal Article
• Authors: Zhou, Zhan; Wang, Qianming; Wang, Jinyong; Zhang, Cheng Cheng
• Is Part Of: Carbon. Volume 93(2015)
• Page Start: 671
• Page End: 680
• Publication Date: 2015-11
• Subjects: Carbon -- Periodicals; Carbone -- Périodiques; Koolstof; Toepassingen; Electronic journals; 546.681
• DOI: 10.1016/j.carbon.2015.05.107
• Languages: English
• ISSNs: 0008-6223

Abstract

Abstract: New chemical sensors based on lanthanide emission that enable sequential detection of two targets in live cells have been developed. The indicator molecules (unsaturated europium complexes) were covalently immobilized on carbon quantum dots (CQDs), and a water soluble fluorescent nano-probe (CQDs-DTPA-Eu ) with biocompatibility was fabricated. A novel stepwise assembly method was used to generate unique responses to two different targets sequentially. Rapid and highly selective detection of tetracycline (Tc) in water and milk (detection limit 32 nM) were demonstrated. The in situ generatedCQDs-DTPA-EuTc was non-emissive, but, upon addition of hydrogen peroxide (detection limit 21 nM) intense red luminescence was observed. Highly reproducible results were obtained in Plat-E cells, demonstrating the identification of tetracycline and H2 O2 in cellular environment with high accuracy.