CCL3, IL‐7, IL‐13 and IFNγ transcripts are increased in skin's biopsy of systemic sclerosis. Issue 10 (3rd July 2019)

• Record Type: Journal Article
• Authors: Gonçalves, Rafaela Silva Guimarães; Pereira, Michelly Cristiny; Dantas, Andréa Tavares; Almeida, Anderson Rodrigues de; Rego, Moacyr J. B. M.; Lima, Emerson Andrade; Pitta, Ivan da Rocha; Duarte, Angela Luzia Branco Pinto; Pitta, Maira Galdino da Rocha
• Other Names: Bauer Johann W. guestEditor.; Bodemer Christine guestEditor.; Schmuth Matthias guestEditor.
• Is Part Of: Experimental dermatology. Volume 28:Issue 10(2019)
• Page Start: 1172
• Page End: 1175
• Publication Date: 2019-07-03
• Subjects: cytokines -- fibrosis -- gene expression profile -- scleroderma; Dermatology -- Periodicals; 616.5
• DOI: 10.1111/exd.13982
• Languages: English
• ISSNs: 0906-6705

Abstract

Abstract: Although several cytokines and chemokines have been investigated as possible mediators of fibrosis in systemic sclerosis (SSc), specific correlation between cytokines and organ involvement have not been found yet, and a cytokine profile characteristic of SSc is far to be identified. We studied the profile of antifibrotic and profibrotic transcripts involved in skin of SSc patients. The mRNA expression was detected by fluorescence‐based quantitative real‐time PCR (qPCR) in skin's biopsies from 14 patients with SSc and 5 healthy controls. PDGF‐A, CTGF, CCL3, IL‐6, IL‐13, IL‐7, IFNγ, IL‐17, IL‐22 and RORc were analysed in these samples. CCL3, IL‐7, IL‐13 and IFN‐γ were more expressed in skin's biopsy of patients with SSc ( P = 0.0002, P = 0.0082, P = 0.0243, P = 0.0335, respectively) when compared with healthy controls. We also found a positive correlation between CCL3 and IL‐7 transcripts ( P = 0.0050 r = 0.7187). Furthermore, we observed that patients with lung involvement had lower expression of PDGF‐A ( P = 0.0385). We found an increase in IL‐7, IFN‐γ, CCL3 and IL‐13 relative mRNA expressions on the skin's biopsy of patients with SSc, and a positive correlation between IL‐7 and CCL3. These molecules are involved in the pathogenesis of SSc, and how their interactions occur should be the subject of further studies.