Streptomyces cyaneochromogenes sp. nov., a blue pigment-producing actinomycete from manganese-contaminated soil. Issue 8 (20th June 2019)

• Record Type: Journal Article
• Authors: Tang, Xinke; Zhao, Jiarong; Li, Kaiqin; Chen, Zhen; Sun, Yuandong; Gao, Jian
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 8(2019)
• Page Start: 2202
• Page End: 2207
• Publication Date: 2019-06-20
• Subjects: Streptomyces cyaneochromogenes sp. nov. -- manganese-contaminated soil -- polyphasic taxonomy; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003406
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel actinomycete isolate, designated strain MK-45 T, was isolated from soil sampled at a manganese-contaminated area in Xiangtan, China. The isolate formed extensively branched substrate mycelia and aerial mycelia that differentiated into tightly coiled or spiral spore chains with smooth-surfaced spores. The cell-wall hydrolysates containedll -diaminopimelic acid and traces of meso -diaminopimelic acid. The major menaquinones consisted of MK-9(H4 ), MK-9(H6 ) and MK-9(H8 ). The major polar lipids contained diphosphatidylgycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphotidylinositol and phosphotidylinositol mannosides. The predominant cellular fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3. Phylogenetic analysis based on the 16S rRNA gene sequences and concatenated partial sequences of the five protein-coding genes indicated that strain MK-45 T was a member of the genus Streptomyces and most closely related to Streptomyces caeruleatus GIMN4.002 T (99.5 % similarity), Streptomyces curacoi CGMCC 4.1680 T (99.5 %), Streptomyces shaanxiensis CCNWHQ 0031 T (99.4 %) and Streptomyces cinnabarinus NRRL B12382 T (98.9 %), respectively. However, the digital DNA–DNA hybridization values, the average nucleotide identity values and MLSA evolutionary distances between strain MK-45 T and them showed that it belonged to a distinct species. Furthermore, the results of morphological, physiological and biochemical tests allowed further phenotypic differentiation of strain MK-45 T from the four closely related type strains mentioned above and other species of the genus Streptomyces with which this strain has 98.0–99.2 % 16S rRNA gene sequence similarity. Therefore, it is concluded that strain MK-45 T represents a novel species of the genus Streptomyces, for which the name Streptomyces cyaneochromogenes sp. nov. is proposed, with MK-45 T (=CICC 11045 T =KCTC 49099 T ) as the type strain.