Legionella qingyii sp. nov., isolated from water samples in China. Issue 7 (7th May 2019)

• Record Type: Journal Article
• Authors: Wu, Hai-Yan; Yan, Hui; Zheng, Min-Ling; Sun, Ming-Ming; Wang, Qun; Hu, Chang-Ming; Zhan, Xiao-Yong; Yuan, Mu-Ge; Qu, Ping-Hua; Hu, Chao-Hui
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 7(2019)
• Page Start: 2017
• Page End: 2022
• Publication Date: 2019-05-07
• Subjects: Legionella qingyii sp. nov. -- polyphasic taxonomy; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003421
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Three Legionella -like strains, designed km488 T, km489 and km521, were isolated from freshwater samples in China. Cells were Gram-stain-negative, rod-shaped and non-spore-forming. Growth was observed on BCYE α agar, but not on BCYE α agar withoutl -cysteine, chocolate agar with PolyViteX or Columbia blood agar. The major fatty acids (>5 %) of strains km488 T, km489 and km521 were C16 : 0, anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 . The mip gene sequences (574 nt) showed the isolates were almost identical with more than 99.7 % sequence similarities, and closely matched to L. gormanii ATCC 33297 T with 95.4–95.6 % sequence similarities. Phylogenetic analyses based on concatenated gene (16S rRNA, mip, rpoB and rnpB ) sequences indicated that the isolates formed a distinct cluster along with L. gormanii within the genus Legionella . Matrix-assisted laser desorption ionization time-of-flight analyses also demonstrated a clear separation between the isolates and other closely and distantly related Legionella species. DNA–DNA hybridization studies demonstrated that the isolates were closely related (92.0 –95.0 % DNA-DNA relatedness) but differentiated from their phylogenetic neighbours (<70 % DNA–DNA relatedness). The whole genome of km488 T was sequenced, and showed a G+C content of 37.8 mol%. Based on the findings from this polyphasic taxonomic study, the isolates are considered to represent a single novel species, for which the name Legionella qingyii sp. nov. is proposed. The type strain is km488 T (KCTC 15636 T =CCTCC AB 2018025 T =NRBC 113223 T ).