Leucobacter muris sp. nov., isolated from the nose of a laboratory mouse. Issue 7 (17th May 2019)

• Record Type: Journal Article
• Authors: Benga, Laurentiu; Spröer, Cathrin; Schumann, Peter; Verbarg, Susanne; Bunk, Boyke; Engelhardt, Eva; Benten, W. Peter M.; Sager, Martin
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 7(2019)
• Page Start: 2095
• Page End: 2100
• Publication Date: 2019-05-17
• Subjects: Leucobacter -- laboratory mouse -- taxonomy; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003446
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-positive, rod-shaped, aerobic, non-motile, white, opaque bacterial isolate, designated 924/12 T, was isolated from the nose of a laboratory mouse in Düsseldorf, Germany. The 16S rRNA gene sequence analyses indicated the phylogenetic position of the strain within the genus Leucobacter . Similarity levels over 97 % were recorded between the 16S rRNA gene sequence of strain 924/12 T and the type strains of the species Leucobacter chironomi DSM 19883 T (99.5 %), followed by Leucobacter celer subsp. astrifaciens CBX151 T (97.6 %), Leucobacter celer subsp. celer NAL101 T (97.5 %), ' Leucobacter kyeonggiensis ' F3-P9 (97.5 %), Leucobacter zeae CC-MF41 T (97.3 %), Leucobacter chromiiresistens JG31 T (97.1 %), Leucobacter triazinivorans JW-1 T (97.1 %), Leucobacter corticis 2 C-7 T (97.0 %) and Leucobacter aridicolis CIP108388 T (97.0 %). DNA–DNA hybridization and whole genomic comparison, mandatory to taxonomically separate strain 924/12 T from the type strain of L. chironomi, revealed similarity values of 40.4 and 30.8 %, respectively, thus below the threshold of 70 % recommended differentiating between species. The cell-wall amino acids of the novel isolate were diaminobutyric acid, alanine, glycine, threonine and glutamic acid. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 . The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipid and one unknown lipid, whereas the predominant menaquinones were MK-11 and MK-10. The genomic DNA G+C content of strain 924/12 T was 70.6 mol%. Phylogenetic analyses based on the 16S rRNA gene sequences and the phenotypical differences between strain 924/12 T and the other closely related type strains of the genus Leucobacter indicated that strain 924/12 T represents a novel species within the genus Leucobacter, family Microbacteriaceae, for which the name Leucobacter muris sp. nov. is proposed. The type strain is 924/12 T (=DSM 101948 T =CCM 8761 T ).