Flavisolibacter nicotianae sp. nov., isolated from rhizosphere soil of Nicotiana tabacum L. Issue 7 (17th May 2019)

• Record Type: Journal Article
• Authors: Li, Yuan-Dong; Zhou, Xing-Kui; Mo, Ming-He; Jiao, Jian-Yu; Yang, Deng-Qiang; Li, Wen-Jun; Zhang, Ti-Kun; Qin, Shi-Chun; Duan, Yan-Qing
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 7(2019)
• Page Start: 2082
• Page End: 2088
• Publication Date: 2019-05-17
• Subjects: Flavisolibacter nicotianae sp. nov. -- polyphasic taxonomy -- rhizosphere soil; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003440
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated strain X7X T, was isolated from a rhizosphere soil sample of Nicotiana tabacum L. collected from a tobacco factory located in Kunming, south-western China. The cells showed oxidase-positive and catalase-positive reactions. Growth occurred at 20–40 °C and pH 6.0–8.0, with optimal growth at 30 °C and pH 7.0. The predominant respiratory quinone was MK-7. The major fatty acids were identified as iso-C15 : 0, iso-C17 : 0 3OH and summed feature 3 (C16 : 1 ω7 c and/or C16 : 1 ω6 c ). The cellular polar lipids contained phosphatidylethanolamine, an unidentified aminophospholipid, two unidentified glycolipids, four unidentified aminolipids and four unidentified lipids. The genomic DNA G+C content was 49.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain X7X T should be affiliated to the genus Flavisolibacter . Results from further analysis showed that strain X7X T had highest 16S rRNA gene sequence similarity to Flavisolibacter metallilatus TX0661 T (96.4 %) and ' Flavisolibacter swuensis ' SR2-4-2 T (96.4 %), followed by other species of the genus Flavisolibacter . The polyphasic taxonomic characteristics indicated that strain X7X T represents a novel species of the genus Flavisolibacter, for which the name Flavisolibacter nicotianae sp. nov. (type strain X7X T =KCTC 62326 T =CGMCC 16451 T ) is proposed.