Bisphenol AF compromises blood-testis barrier integrity and sperm quality in mice. (December 2019)

• Record Type: Journal Article
• Authors: Wu, Di; Huang, Chun-Jie; Jiao, Xiao-Fei; Ding, Zhi-Ming; Zhang, Shou-Xin; Miao, Yi-Liang; Huo, Li-Jun
• Is Part Of: Chemosphere. Volume 237(2019)
• Publication Date: 2019-12
• Subjects: Bisphenol AF -- Blood-testis barrier -- Cytoskeleton architecture -- Spermatogenesis -- Tight junction -- MAPK; EDCs Endocrine-disrupting chemicals -- BPA Bisphenol A -- BPAF Bisphenol AF -- GPER G protein-coupled estrogen receptor -- ER estrogen receptor -- ROS reactive oxygen species -- HCA high-content analysis -- BTB Blood-testis barrier -- TJ tight junction -- basal ES basal ectoplasmic specialization -- GJ gap junction -- MAPKs mitogen-activated protein kinases -- H3K27me3 histone H3 Lys27 trimethylation -- GPX4 Glutathione peroxidase 4 -- SDHB Succinate dehydrogenase -- PRDX5 Peroxiredoxin 5 -- FITC-PNA fluorescein isothiocyanate-conjugated peanut agglutinin -- PTMs post-translational modifications -- TER trans-epithelial electrical resistance -- TNPs transition nuclear proteins -- RNE redundant nuclear envelope -- DRP1 dynamin-related protein 1 -- UPS ubiquitination-proteasome system -- EZH2 enhancer of Zeste Homolog 2; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2019.124410
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: The profound influence of environmental chemicals on human health including inducing life-threatening gene mutation has been publicly recognized. Being a substitute for the extensively used endocrine-disrupting chemical BPA, Bisphenol AF (BPAF) has been known as teratogen with developmental toxicities and therefore potentially putting human into the risk of biological hazards. Herein, we deciphered the detrimental effects of BPAF on spermatogenesis and spermiotiliosis in sexual maturity of mice exposing to BPAF (5, 20, 50 mg/kg/d) for consecutive 28 days. BPAF exposure significantly compromises blood-testis barrier integrity and sperm quantity and quality in a dose-dependent manner. Sperms from BPAF exposure mice are featured by severe DNA damage, altered SUMOylation and ubiquitination dynamics and interfered epigenetic inheritance with hypermethylation of H3K27me3 presumably due to the aggregation of cellular reactive oxygen species (ROS). Furthermore, BPAF treatment (50 μM for 24 h) compromises cytoskeleton architecture and tight junction permeability in primary cultured Sertoli cells evidenced by dysfunction of actin regulatory proteins (e.g. Arp3 and Palladin) via activation of ERK signaling, thereby perturbing the privilege microenvironment created by Sertoli cells for spermatogenesis. Overall, our study determines BPAF is deleterious for male fertility, leading to a better appreciation of its toxicological features in our life. Highlights: BPAF exposure compromises sperm production and quality and disrupts the post-translational modifications of sperm. BPAF exposure significantly compromises blood-testis barrier integrity both in vivo and in vitro. BPAF perturbs the cytoskeleton architecture via activation of ERK1/2 signaling.