Diagnostic markers selected by immunoproteomics and phage display applied for the serodiagnosis of canine leishmaniosis. (October 2019)

• Record Type: Journal Article
• Authors: Machado, Juliana M.; Costa, Lourena E.; Dias, Daniel S.; Ribeiro, Patricia A.F.; Martins, Vívian T.; Lage, Daniela P.; Carvalho, Gerusa B.; Franklin, Michelle L.; Tavares, Grasiele S.V.; Oliveira-da-Silva, João A.; Machado, Amanda S.; Ramos, Luana S.; Nogueira, Lais M.; Mariano, Reysla M.S.; Moura, Henrique B.; Silva, Eduardo S.; Teixeira-Neto, Rafael G.; Campos-da-Paz, Mariana; Galdino, Alexsandro S.; Coelho, Eduardo A.F.
• Is Part Of: Research in veterinary science. Volume 126(2019)
• Page Start: 4
• Page End: 8
• Publication Date: 2019-10
• Subjects: Serodiagnosis -- Canine leishmaniosis -- Immunoproteomics -- Hypothetical proteins -- Phage display -- Mimotopes; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2019.08.010
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: Canine leishmaniosis (CanL) is one of the most important parasitic diseases found in several countries worldwide. Dogs are considered important domestic reservoirs of the parasites, being relevant in the maintenance of transmission cycle of the disease between sandflies and humans. However, the prevalence of asymptomatic infection is considerably higher than that of apparent clinical illness in the infected animals; thus making promptly necessary to diagnose the infection in these animals, which could help to allow to the adoption of more efficient control measures against disease. Parasitological tests, which are considered as gold standard to demonstrate the infection and diagnose the disease, present problems related with their sensitivity. Also, the sample´s collect is considered invasive. As consequence, serological tests could be applied as an additional tool to detect the asymptomatic and symptomatic CanL. For this purpose, distinct recombinant antigens have been studied; however, problems in their sensitivity and/or specificity have been still registered. The present review focus in advances in the identification of new diagnostic targets applied for the CanL diagnose, represented here by recombinant single, combined or chimeric proteins, as well as by peptides that mimic epitopes (mimotopes); which were selected by means of immunoproteomics and phage display. Highlights: A rapid and precise diagnosis for canine leishmaniosis is desirable. New recombinant antigens have been tested for the serodiagnosis of disease. Such antigenic markers showed variable sensitivity and/or specificity. New diagnostic targets were identified by immunoproteomics and phage display. This work focuses in such antigenic molecules identified by these technologies.