Pseudogemmobacter bohemicus gen. nov., sp. nov., a novel taxon from the Rhodobacteraceae family isolated from heavy-metal-contaminated sludge. Issue 8 (5th June 2019)

• Record Type: Journal Article
• Authors: Suman, Jachym; Zubrova, Andrea; Rojikova, Katerina; Pechar, Radko; Svec, Pavel; Cajthaml, Tomas; Ulbrich, Pavel; Ridl, Jakub; Strnad, Hynek; Uhlik, Ondrej
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 8(2019)
• Page Start: 2401
• Page End: 2407
• Publication Date: 2019-06-05
• Subjects: Rhodobacteraceae -- Pseudogemmobacter bohemicus -- 16S rRNA gene analysis -- chemotaxonomic characterization -- percentage of conserved proteins -- heavy metals; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003493
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : The creamy white to beige, aerobic, non-motile, ovoid to rod-shaped, Gram-stain-negative strain, Cd-10 T, was isolated from heavy-metal-contaminated sludge from a decantation basin of a heavy metal processing factory based on its ability to tolerate CdCl2 in the cultivation medium. In the reconstruction of its phylogeny based on 16S rRNA gene sequences, strain Cd-10 T clustered with species of the genera Gemmobacter, Xinfangfangia, Tabrizicola and Rhodobacter within the family Rhodobacteraceae . Its 16S rRNA gene sequence exhibited 96.32 % pairwise similarity to the type strain of Xinfangfangia soli, 95.3 % to that of Gemmobacter intermedius, followed by Tabrizicola fusiformis (95.10 %), Rhodobacter sediminis (94.88 %), Gemmobacter nectariphilus and Rhodobacter capsulatus (both 94.81 %). The major respiratory quinone was Q-10 accompanied by Q-9, the fatty acid profile consisted predominantly of C18 : 1 ω7 c, C18 : 0, C16 : 0 and C16 : 1 ω7 c, the major polar lipids were phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine and diphosphatidylglycerol. An analysis of the percentage of conserved proteins deduced from draft or complete genomic sequences of strain Cd-10 T and representatives of its closest relatives suggested that strain Cd-10 T is a member of a novel genus within the Rhodobacteraceae family for which we propose the name Pseudogemmobacter . Strain Cd-10 T (=DSM 103618 T =NCCB 100645 T ) is the type strain of Pseudogemmobacter bohemicus gen. nov., sp. nov., the type species of the genus Pseudogemmobacter gen. nov.