Pulveribacter suum gen. nov., sp. nov., isolated from a pig farm dust collector. Issue 7 (2nd May 2019)

• Record Type: Journal Article
• Authors: Heo, Jun; Cho, Ha-Young; Heo, Inbeom; Hong, Seung-Bum; Kim, Jeong-Seon; Kwon, Soon-Wo; Kim, Soo-Jin
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 7(2019)
• Page Start: 1864
• Page End: 1869
• Publication Date: 2019-05-02
• Subjects: Pulveribacter suum -- taxonomy -- new taxa -- dust collector; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003082
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : An aerobic, Gram-stain-negative, polar-flagellated, rod-shaped bacterium, designated as SC2-7 T, was isolated from the dust collector at a pig farm located in Wanju-gun, Jeollabuk-do, Republic of Korea. Growth occurred at 10–37 °C (optimum, 28–30 °C), pH 6.0–10.0 (optimum, 7.0–8.0) and in the presence of 0–3 % (w/v) NaCl (optimum, 0 %) on Reasoner's 2A medium. The phylogenetic tree based on the 16S rRNA gene sequences revealed that strain SC2-7 T was a member of the family Comamonadaceae, forming a robust cluster with the genera Alicycliphilus, Oryzisolibacter and Melaminivora . The 16S rRNA gene sequences of strain SC2-7 T showed the highest sequence similarities to Alicycliphilus denitrificans K601 T (97.2 %), Oryzisolibacter propanilivorax EPL6 T (97.0 %), Melaminivora alkalimesophila CY1 T (96.9 %), Diaphorobacter polyhydroxybutyrativorans SL-205 T (96.6 %), Diaphorobacter nitroreducens NA10B T (96.6 %) and Melaminivora jejuensis KBB12 T (96.5 %). The tree based on the gyrA gene sequences also showed that strain SC2-7 T fell into a phylogenetic position similar to that based on the 16S rRNA gene sequences. The polar lipids present were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminolipid and an unidentified phospholipid. The predominant quinone was ubiquione-8. The major fatty acids were summed feature 3 (including C16 : 1 ω6 c and/or C16 : 1 ω7 c ), C16 : 0 and summed feature 8 (including C18 : 1 ω6 c and/or C18 : 1 ω 7 c ). The genomic DNA G+C content was 69.1 mol%. On the basis of the phenotypic, phylogenetic and chemotaxonomic data presented here, strain SC2-7 T represents a novel species of a new genus, for which the name Pulveribacter suum gen. nov., sp. nov., is proposed. The type strain of Pulveribacter suum is SC2-7 T (=KACC 19309 T =NBRC 113102 T ).