Α‐Synuclein RT‐QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies. Issue 10 (10th October 2019)

• Record Type: Journal Article
• Authors: Bongianni, Matilde; Ladogana, Anna; Capaldi, Stefano; Klotz, Sigrid; Baiardi, Simone; Cagnin, Annachiara; Perra, Daniela; Fiorini, Michele; Poleggi, Anna; Legname, Giuseppe; Cattaruzza, Tatiana; Janes, Francesco; Tabaton, Massimo; Ghetti, Bernardino; Monaco, Salvatore; Kovacs, Gabor G.; Parchi, Piero; Pocchiari, Maurizio; Zanusso, Gianluigi
• Is Part Of: Annals of clinical and translational neurology. Volume 6:Issue 10(2019)
• Page Start: 2120
• Page End: 2126
• Publication Date: 2019-10-10
• Subjects: Nervous system -- Diseases -- Periodicals; Neurology -- Periodicals; 616.8005
• DOI: 10.1002/acn3.50897
• Languages: English
• ISSNs: 2328-9503

Abstract

Abstract: We applied RT‐QuIC assay to detect α‐synuclein aggregates in cerebrospinal fluid (CSF) of patients with suspected Creutzfeldt–Jakob disease who had a neuropathological diagnosis of dementia with Lewy bodies (DLB) ( n = 7), other neurodegenerative diseases with α‐synuclein mixed pathology ( n = 20), or without Lewy‐related pathology ( n = 49). The test had a sensitivity of 92.9% and specificity of 95.9% in distinguishing α‐synucleinopathies from non‐α‐synucleinopathies. When performed in the CSF of patients with DLB ( n = 36), RT‐QuIC was positive in 17/20 with probable DLB, 0/6 with possible DLB, and 0/10 with Alzheimer disease. These results indicate that RT‐QuIC for α‐synuclein is an accurate test for DLB diagnosis.