Screening of acetylcholinesterase inhibitors in marine organisms from the Caribbean Sea. Issue 24 (17th December 2019)

• Record Type: Journal Article
• Authors: Castellanos, Fabio; Amaya-García, Fabián; Tello, Edisson; Ramos, Freddy Alejandro; Umaña, Adriana; Puyana, Mónica; Resende, Jackson A. L. C.; Castellanos, Leonardo
• Is Part Of: Natural product research. Volume 33:Issue 24(2019)
• Page Start: 3533
• Page End: 3540
• Publication Date: 2019-12-17
• Subjects: Acetylcholinesterase inhibitors -- marine bioprospecting -- cembranes -- marine natural products -- Eunicea knigthi -- Pseudoplexaura porosa; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2018.1481837
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: The acetylcholinesterase inhibitory activity of 89 organic extracts from marine organisms was evaluated through a TLC bioautography methodology. Extracts from soft corals ( Eunicea and Plexaura ) were the most active compared with extracts from sponges. The bioguided chemical study of the most active extract, obtained from Pseudoplexaura porosa, led to the isolation of a diterpene with spectroscopic properties consistent to those published to the cembrane Steylolide. However, further analysis by X-ray diffraction indicated that the compound was the 14-acetoxycrassine (1 ), correcting the structure reported to the Styelolide. Additionally, the acetylcholinesterase inhibitory activity of fourteen cembranoids (2 –15 ) isolated from soft corals Eunicea knighti and Pseudoplexaura flagellosa was evaluated. Cembranoids2, 3 and4 were the most active compounds in the TLC bioassay. Then, the most promising cembranoids, 14-acetoxycrassine (1 ) and asperdiol (2 ), were tested quantitatively and they exhibited IC50 values of 1.40 ± 0.113 and 0.358 ± 0.130 μM, respectively. Abstract :