A novel turn-on fluorescent probe for Hg2+ detection based on rhodamine B spirolactam derivative. Issue 15 (8th December 2019)

• Record Type: Journal Article
• Authors: Dan, Wen; Deng, Xuankai; Yu, Yanhua
• Is Part Of: International journal of environmental analytical chemistry. Volume 99:Issue 15(2019)
• Page Start: 1515
• Page End: 1527
• Publication Date: 2019-12-08
• Subjects: Rhodamine -- Hg2+ detection -- fluorescence turn-on -- test strip; Ecology -- Periodicals; Chemistry, Analytic -- Periodicals; Environmental chemistry -- Periodicals; Pollution -- Periodicals; 577.14
• DOI: 10.1080/03067319.2019.1625339
• Languages: English
• ISSNs: 0306-7319

Abstract

ABSTRACT: In this work, a new turn-on fluorescent probe1 for Hg 2+ ions detection based on rhodamine B spirolactam was reported. Among tested metal ions, probe1 shows high selectivity towards Hg 2+ in the the mixture solution of methanol and 0.02 M HEPES buffer (V/V = 9:1, pH = 7.2). No absorption and emission band of probe1 was observed in the range from 450 to 700 nm. While only addition of Hg 2+ to probe1 could lead to appearance of a new absorption band centered at 553 nm and a fluorescence emission band around 577 nm upon excitation at 520 nm. Moreover, it exhibits excellent linear relationship (R 2 = 0.9993) between fluorescence intensity at 577 nm and the concentration of Hg 2+ from 1.6 to 32 μM. The sensing mechanism was proven to be spirolactam ring open induced by Hg 2+ through 1 H NMR, MS, absorption and fluorescence spectra. In addition, probe1 could detect Hg 2+ in real water samples and on filter paper, which demonstrates its application in environment science.