Regulation of the transcription factor E2F8 gene expression in bovine ovarian cells. (1st December 2019)

• Record Type: Journal Article
• Authors: Morrell, Breanne C.; Zhang, Lingna; Schütz, Luis F.; Perego, M. Chiara; Maylem, Excel Rio S.; Spicer, Leon J.
• Is Part Of: Molecular and cellular endocrinology. Volume 498(2019)
• Publication Date: 2019-12-01
• Subjects: Granulosa cell -- theca cell -- E2F8 transcription factor -- Cattle; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2019.110572
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: Overexpression of the transcription factor, E2F8, has been associated with ovarian cancer. Objectives of this study were to determine: 1) if E2F8 gene expression in granulosa cells (GC) and theca cells (TC) change with follicular development, and 2) if E2F8 mRNA abundance in TC and GC is hormonally regulated. Using real-time PCR, E2F8 mRNA abundance in GC and TC was greater ( P < 0.05) in small than large follicles. FGF9 induced an increase ( P < 0.05) in E2F8 mRNA abundance by 1.6- to 7-fold in large-follicle (8–20 mm) TC and GC as well as in small-follicle (1–5 mm) GC. Abundance of E2F8 mRNA in TC was increased ( P < 0.05) with FGF2, FGF9 or VEGFA treatments alone in vitro, and concomitant treatment of VEGFA with FGF9 increased ( P < 0.05) abundance of E2F8 mRNA above any of the singular treatments; BMP4, WNT3A and LH were without effect. IGF1 amplified the stimulatory effect of FGF9 on E2F8 mRNA abundance by 2.7-fold. Collectively, our studies show for the first time that follicular E2F8 is developmentally and hormonally regulated indicating that E2F8 may be involved in follicular development. Highlights: E2F8 mRNA abundance is greater in small versus large follicles. E2F8 mRNA in theca cells and granulosa cells was induced by FGF9. E2F8 mRNA induced by FGF9 in theca cells is enhanced by IGF1 and VEGFA. E2F8 may be involved in follicular growth of cattle.