The increase of amino acids induces the expression of vitellogenin after spinning in the silkworm Bombyx mori. (October 2019)

• Record Type: Journal Article
• Authors: Liu, Hongling; Lin, Ying; Gu, Jianjian; Ruan, Yang; Shen, Guanwang; Zhang, Yujing; Wang, Huijuan; Meng, Ziwang; Li, Kairong; Xia, Qingyou
• Is Part Of: Journal of insect physiology. Volume 118(2019)
• Publication Date: 2019-10
• Subjects: Silkworm -- Amino acids -- Regulation -- Vitellogenin -- Expression; Insects -- Physiology -- Periodicals; Insectes -- Physiologie -- Périodiques; Insects -- Physiology; Periodicals; 571.157
• DOI: 10.1016/j.jinsphys.2019.103913
• Languages: English
• ISSNs: 0022-1910

Abstract

Graphical abstract: Highlights: Amino acid content increases upon knockout of Fib-H . The majority of the main amino acids of silk protein could induce BmVg expression. Amino acid induces BmVg expression through the TOR signalling pathway. AA injection induces BmVg up-regulation after finishing spinning. Abstract: Silkworms are economically important insects because of the value of their silk. After finishing silk spinning, silkworms begin another important physiological process, vitellogenesis. In this study, we explored the relationship between silk spinning and vitellogenin ( BmVg ) expression in silkworms. In silkworms with the silk fibroin heavy chain gene knocked-out, the concentration of amino acids in the hemolymph was found to be significantly higher than that in the wild type, and the expression of BmVg was advanced at day 7 of the fifth instar stage and 0 h after spinning. Furthermore, through culturing fat body in vitro with different substances treatment including glucose, trehalose, amino acids, 20-hydroxyecdysone, and insulin, we found that only amino acids could induce BmVg expression. RNA interference of BmTOR1 in female silkworms could down-regulate BmVg transcription, resulting in shortened egg ducts and smaller eggs relative to the control. Therefore, these results showed that amino acids could induce BmVg expression through the TOR signaling pathway. Fat body cultured with amino acids in vitro and experiments involving amino acids injected into the silkworm showed that the majority of main amino acids of silk protein could induce BmVg expression. These results suggested that BmVg expression is related to silk spinning and this study would lay a foundation for elucidating the stage specificity expression of BmVg .