Realizing the potential of full-length transcriptome sequencing. (7th October 2019)

• Record Type: Journal Article
• Authors: Byrne, Ashley; Cole, Charles; Volden, Roger; Vollmers, Christopher
• Is Part Of: Philosophical transactions. Volume 374:Number 1786(2019)
• Publication Date: 2019-10-07
• Subjects: transcriptome analysis -- long-read sequencing -- Oxford Nanopore Technologies -- Pacific Biosciences; Biology -- Periodicals; Science -- Periodicals; 570
• DOI: 10.1098/rstb.2019.0097
• Languages: English
• ISSNs: 0962-8436

Abstract

Abstract : Long-read sequencing holds great potential for transcriptome analysis because it offers researchers an affordable method to annotate the transcriptomes of non-model organisms. This, in turn, will greatly benefit future work on less-researched organisms like unicellular eukaryotes that cannot rely on large consortia to generate these transcriptome annotations. However, to realize this potential, several remaining molecular and computational challenges will have to be overcome. In this review, we have outlined the limitations of short-read sequencing technology and how long-read sequencing technology overcomes these limitations. We have also highlighted the unique challenges still present for long-read sequencing technology and provided some suggestions on how to overcome these challenges going forward. This article is part of a discussion meeting issue 'Single cell ecology'.