A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives. (1st November 2018)

• Record Type: Journal Article
• Authors: Parkhouse, R Michael E; Carpio, Arturo; Campoverde, Alfredo; Sastre, Patricia; Rojas, Glenda; Harrison, Leslie J S; Cortez, Maria Milagros
• Is Part Of: Transactions of the Royal Society of Tropical Medicine and Hygiene. Volume 113:Number 2(2019)
• Page Start: 101
• Page End: 104
• Publication Date: 2018-11-01
• Subjects: bovine cysticercosis -- field diagnosis -- HP10 antigen ELISA -- HP10 antigen LFA -- neurocysticercosis (NCC); Tropical medicine -- Periodicals; 616.9883
• DOI: 10.1093/trstmh/try116
• Languages: English
• ISSNs: 0035-9203

Abstract

Abstract: Background: Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods: Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results: The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was marginally more sensitive than the modified HP10 Ag-LFA. Conclusions: The modified HP10 Ag-LFA provides a field test for the rapid identification of endemic human and bovine cysticercosis.