MiR‐9 promotes multiple myeloma progression by regulating TRIM56/NF‐κB pathway. (17th July 2019)

• Record Type: Journal Article
• Authors: Huang, Guoqiang; Liu, Xiaopeng; Zhao, Xiaoying; Zhao, Jing; Hao, Jinxia; Ren, Juan; Chen, Ying
• Is Part Of: Cell biology international. Volume 43:Number 11(2019)
• Page Start: 1223
• Page End: 1233
• Publication Date: 2019-07-17
• Subjects: miR‐9 -- multiple myeloma -- NF‐κB pathway -- tripartite motif‐containing protein 56 (TRIM56); Cytology -- Periodicals; Cells -- Periodicals; 571.605
• DOI: 10.1002/cbin.11104
• Languages: English
• ISSNs: 1065-6995

Abstract

Abstract: miR‐9 has been reported to play a pivotal role in multiple human cancers by acting as an oncogene or tumor suppressor. In this study, we explored the possible role and molecular mechanism of miR‐9 in multiple myeloma (MM). The miR‐9 expression was examined by quantitative real‐time polymerase chain reaction assay. Transfection with miR‐9‐mimics, miR‐9‐inhibitor, pcDNA‐TRIM56, or si‐TRIM56 into cells was used to change the expression levels of miR‐9 and TRIM56. Western blot analysis was used to detect the expression of TRIM56, p65, p‐p65, IκBα, and p‐IκBα. The potential target of miR‐9 was confirmed by luciferase reporter assay. The 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyltetrazolium (MTT) assay, colony formation assay, and flow cytometry were used to assess the abilities of cell proliferation and apoptosis. miR‐9 was upregulated in MM patients and cell lines, and miR‐9 overexpression promoted proliferation and repressed apoptosis in MM cell lines. TRIM56 was confirmed as a target of miR‐9. Moreover, TRIM56 reversed miR‐9‐mediated pro‐proliferation and anti‐apoptosis effect on MM cell lines. Furthermore, nuclear factor‐κB (NF‐κB) pathway was involved in miR‐9/TRIM56‐mediated regulation on MM cell lines. miR‐9 promoted the development and progression of MM by regulating TRIM56/NF‐κB pathway, thereby providing a potential microRNA‐based target for MM therapy.