Structural insights into repression of the Pneumococcal fatty acid synthesis pathway by repressor FabT and co‐repressor acyl‐ACP. Issue 19 (30th July 2019)

• Record Type: Journal Article
• Authors: Zuo, Gang; Chen, Zhi‐Peng; Jiang, Yong‐Liang; Zhu, Zhongliang; Ding, Chengtao; Zhang, Zhiyong; Chen, Yuxing; Zhou, Cong‐Zhao; Li, Qiong
• Is Part Of: FEBS letters. Volume 593:Issue 19(2019)
• Page Start: 2730
• Page End: 2741
• Publication Date: 2019-07-30
• Subjects: co‐repressor -- fatty acid synthesis -- repression -- transcription repressor; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.13534
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : The Streptococcus pneumoniae fatty acid synthesis (FAS) pathway is globally controlled at the transcriptional level by the repressor FabT and its co‐repressor acyl carrier protein (acyl‐ACP), the intermediate of phospholipid synthesis. Here, we report the crystal structure of FabT complexed with a 23‐bp dsDNA, which indicates that FabT is a weak repressor with low DNA‐binding affinity in the absence of acyl‐ACP. Modification of ACP with a long‐chain fatty acid is necessary for the formation of a stable complex with FabT, mimicked in vitro by cross‐linking, which significantly elevates the DNA‐binding affinity of FabT. Altogether, we propose a putative working model of gene repression under the double control of FabT and acyl‐ACP, elucidating a distinct repression network for Pneumococcus to precisely coordinate FAS. Abstract :