A gamma‐thionin protein from apple, MdD1, is required for defence against S‐RNase‐induced inhibition of pollen tube prior to self/non‐self recognition. Issue 11 (17th May 2019)

• Record Type: Journal Article
• Authors: Gu, Zhaoyu; Li, Wei; Doughty, James; Meng, Dong; Yang, Qing; Yuan, Hui; Li, Yang; Chen, Qiuju; Yu, Jie; Liu, Chun sheng; Li, Tianzhong
• Is Part Of: Plant biotechnology journal. Volume 17:Issue 11(2019)
• Page Start: 2184
• Page End: 2198
• Publication Date: 2019-05-17
• Subjects: Malus domestica -- MdD1 -- pollen tube growth -- plant defence -- S‐RNase; Plant biotechnology -- Periodicals; Plant genetic engineering -- Periodicals; 630.272
• DOI: 10.1111/pbi.13131
• Languages: English
• ISSNs: 1467-7644

Abstract

Summary: Apple exhibits S ‐RNase‐mediated self‐incompatibility. Although the cytotoxic effect of S ‐RNase inside the self‐pollen tube has been studied extensively, the underlying defence mechanism in pollen tube in Rosaceae remains unclear. On exposure to stylar S ‐RNase, plant defence responses are activated in the pollen tube; however, how these are regulated is currently poorly understood. Here, we show that entry of both self and non‐self S ‐RNase into pollen tubes of apple ( Malus domestica ) stimulates jasmonic acid (JA) production, in turn inducing the accumulation of MdMYC2 transcripts, a transcription factor in the JA signalling pathway widely considered to be involved in plant defence processes. MdMYC2 acts as a positive regulator in the pollen tube activating expression of MdD1, a gene encoding a defence protein. Importantly, MdD1 was shown to bind to the RNase activity sites of S ‐RNase leading to inhibition of enzymatic activity. This work provides intriguing insights into an ancient defence mechanism present in apple pollen tubes where MdD1 likely acts as a primary line of defence to inhibit S ‐RNase cytotoxicity prior to self/non‐self recognition.