Cardiovascular effects of diesel exhaust inhalation: photochemically altered versus freshly emitted in mice. Issue 17 (2nd September 2019)

• Record Type: Journal Article
• Authors: Tong, Haiyan; Zavala, Jose; McIntosh-Kastrinsky, Rachel; Sexton, Kenneth G.
• Is Part Of: Journal of toxicology and environmental health. Volume 82:Issue 17(2019)
• Page Start: 944
• Page End: 955
• Publication Date: 2019-09-02
• Subjects: Diesel exhaust -- photochemistry -- cardiac function; Toxicology -- Periodicals; Environmental health -- Periodicals; 615.90205
• DOI: 10.1080/15287394.2019.1671278
• Languages: English
• ISSNs: 1528-7394

Abstract

ABSTRACT: This study was designed to compare the cardiovascular effects of inhaled photochemically altered diesel exhaust (aged DE) to freshly emitted DE (fresh DE) in female C57Bl/6 mice. Mice were exposed to either fresh DE, aged DE, or filtered air (FA) for 4 hr using an environmental irradiation chamber. Cardiac responses were assessed 8 hr after exposure utilizing Langendorff preparation with a protocol consisting of 20 min of perfusion and 20 min of ischemia followed by 2 hr of reperfusion. Cardiac function was measured by indices of left-ventricular-developed pressure (LVDP) and contractility (dP/dt) prior to ischemia. Recovery of post-ischemic LVDP was examined on reperfusion following ischemia. Fresh DE contained 460 µg/m 3 of particulate matter (PM), 0.29 ppm of nitrogen dioxide (NO2 ) and no ozone (O3 ), while aged DE consisted of 330 µg/m 3 of PM, 0.23 ppm O3 and no NO2 . Fresh DE significantly decreased LVDP, dP/dtmax, and dP/dtmin compared to FA. Aged DE also significantly reduced LVDP and dP/dtmax . Data demonstrated that acute inhalation to either fresh or aged DE lowered LVDP and dP/dt, with a greater fall noted with fresh DE, suggesting that the composition of DE may play a key role in DE-induced adverse cardiovascular effects in female C57Bl/6 mice.