Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model. (November 2019)

• Record Type: Journal Article
• Authors: Nešić, Andrijana; Stam, Annemarie; Čavić, Milena; Ten Klooster, Jean Paul; Pieters, Raymond; Smit, Joost; Gavrović-Jankulović, Marija
• Is Part Of: Journal of functional foods. Volume 62(2019)
• Publication Date: 2019-11
• Subjects: Act d 1 -- Cysteine protease -- Food allergy -- Pro-inflammatory cytokines -- TJ proteins; Act d 1 actinidin -- IgE immunoglobulin E -- TJ tight junction -- GIT gastrointestinal tract -- CLDN claudin -- OCLN occludin -- ZO zonula ocludens -- IL-1β interleukin 1β -- TNFα tumor necrosis factor α -- IL-33 interleukin 33 -- IL-25 interleukin 25 -- TSLP thymic stromal lymphopoietin -- TEER transepithelial electrical resistance -- FCS fetal calf serum -- BLG β-Lactoglobulin -- PCR polymerase chain reaction -- ELISA enzyme-linked immunosorbent assay -- ANOVA analysis of variance -- GAPDH glyceraldehyde 3-phosphate dehydrogenase -- ACTB β-actin; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2019.103556
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Act d 1 up-regulates tight junction proteins in Caco-2 monolayers. Act d 1 up-regulates tight junction proteins in 2-Dimensional intestinal organoids. Act d 1 induces release of proinflamatory cytokines in 2D organoids and Caco-2 cells. Act d 1 impaired TJs network of E-cadherin, claudin-3 and ZO-1 in 2D-organoids. 2D mouse organoids are a promising model for assessment of intestinal cell response. Abstract: In this study, two intestinal models were employed to assess the modulatory potential of a major kiwifruit allergen on the innate immunity of epithelial cells. Effects of Act d 1 were analyzed in terms of gene expression and structural changes of tight junction (TJ) proteins, as well as up-regulation of pro-inflammatory cytokines in Caco-2 cells and, for the first time, in mouse-derived intestinal 2-dimensional (2D) organoids. Biologically active Act d 1 induced up-regulation of TJ genes for CLDN-2, CLDN-3, CLDN-4, ZO-1, and on the protein level induced release of pro-inflammatory cytokines IL-1β, TNFα and IL-33 in both employed model systems. In 2D-organoids, active Act d 1 impaired the TJ protein networks of E-cadherin, claudin-3, and ZO-1. 2D-organoids generated from mouse intestine are a promising new model system for the assessment of allergen-induced intestinal cell responses and a useful tool for mitigation of risks associated with novel food proteins.