Cyclic tensile force-upregulated IL6 increases MMP3 expression by human periodontal ligament cells. (November 2019)

• Record Type: Journal Article
• Authors: Tantilertanant, Yanee; Niyompanich, Jitti; Everts, Vincent; Supaphol, Pitt; Pavasant, Prasit; Sanchavanakit, Neeracha
• Is Part Of: Archives of oral biology. Volume 107(2019)
• Publication Date: 2019-11
• Subjects: Cyclic tensile force -- Human periodontal ligament cells -- IL6 -- MMP3; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2019.104495
• Languages: English
• ISSNs: 0003-9969

Abstract

Highlights: CTF induces synthesis of IL6 and MMP3 by human PDL cells. CTF-upregulated IL6 increases synthesis of MMP3. IL6 induces MMP3 expression via PI3K and MAPK signaling pathways. Abstract: Objective: Cyclic tensile force (CTF) modulates physiological responses of periodontal ligament (PDL) cells. PDL cells are mechanosensitive and are able to maintain tissue homeostasis; a process mediated by the expression of particular cytokines including interleukin 6 (IL6). It is unknown whether CTF-induced IL6 regulates the expression of MMPs, enzymes needed for tissue remodeling. Design: Human PDL cells were subjected to 10% elongation strain of CTF at a frequency of 60 rpm continuously for 6 h. RNA and proteins were extracted and analyzed for IL6 and MMP expression by quantitative real-time PCR and ELISA, respectively. Using a neutralizing anti-IL6 antibody and addition of recombinant human IL6 at concentrations of 0.1, 1, 10 ng.mL −1 were performed to clarify whether CTF-upregulated IL6 increased MMP expression. Inhibitors of intracellular signaling molecules were employed to reveal possible pathway(s) of IL6-induced MMP expression. Results: CTF-induced IL6 expression coincided with an increased MMP3 expression. A neutralizing anti-IL6 antibody attenuated the CTF-increased MMP3 expression, whereas stimulating the cells with recombinant human IL6 increased MMP3 expression. Both PI3K and MAPK pathways were essential in the IL6 induced expression of MMP3 . Conclusion: Our findings suggest a role of CTF in the modulation of expression of IL6 and MMP3 and thus in the regulation of homeostasis and remodeling of the periodontal ligament.