The anti-oxidative and hemostasis-related multifunctionality of L-chicoric acid, the main component of dandelion: An in vitro study of its cellular safety, antioxidant and anti-platelet properties, and effect on coagulation. (November 2019)

• Record Type: Journal Article
• Authors: Lis, Bernadetta; Jedrejek, Dariusz; Moldoch, Jaroslaw; Stochmal, Anna; Olas, Beata
• Is Part Of: Journal of functional foods. Volume 62(2019)
• Publication Date: 2019-11
• Subjects: Antiplatelet activity -- Adhesion -- Aggregation -- Blood platelets -- Chicoric acid -- Dandelion; ADP adenosine diphosphate -- APTT activated partial thromboplastin time -- BSA bovine serum albumin -- CPDA citrate/phosphate/dextrose/adenine -- CVDs cardiovascular diseases -- DHBV duck hepatitis B virus -- DMSO dimethylsulfoxide -- EDTA ethylenediaminetetraacetic acid -- Fg fibrinogen -- fraction A leaf 50% MeOH fraction -- fraction B leaf 85% MeOH fraction -- fraction C petal 50% MeOH fraction -- fraction D petal 85% MeOH fraction -- GSH glutathione -- HBV Hepatitis B virus -- HIV Human Immunodeficiency Virus -- 5-HT 5-hydroxytryptamine -- LDH lactate dehydrogenase -- NADH nicotinamide-adenine-dinucleotide -- O2− superoxide anion -- PCR polymerase chain reaction -- PDGF platelet-derived growth factor -- PF4 platelet factor 4 -- PRP platelet-rich plasma -- ROS reactive oxygen species -- PT prothrombin time -- TBARS thiobarbituric acid reactive substances -- Tsp thrombospondin -- TT thrombin time -- TXA2 thromboxane A2 -- vWf von Willebrand factor -- WHO World Health Organization; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2019.103524
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Chicoric acid has different biological properties without cytotoxicity. Dandelion leaves are a rich source of chicoric acid. 50% fraction from dandelion leaves had the best anti-platelet activity. Chicoric acid did not change coagulation times of human plasma. Abstract: All dandelion ( Taraxacum officinale ) organs, i.e. the roots, leaves and flowers, are rich in L-chicoric acid, and nowadays are commonly included in dietary products and pharmaceutical preparations. However, little is known of the effects of dandelion leaves and petals, or L-chicoric acid itself, on the biological activity hemostasis elements such as blood platelets and plasma. Therefore, the aim of the present study was to examine the effect of chicoric acid extracted from dandelion on the biological properties of human plasma and blood platelets in vitro . Briefly, the study evaluated the effects of four phenolic fractions, obtained from leaves (fraction A and B) and petals (fraction C and D), with different concentrations of chicoric acid, as well as L-chicoric acid isolated from dandelion leaves, on biomarkers of oxidative stress, parameters of coagulation and blood platelet activation. Chicoric acid offers novel antioxidant and anti-adhesive potential without cytotoxicity.