Levan production from sucrose using chicken feather peptone as a low cost supplemental nutrient source. (1st January 2020)

• Record Type: Journal Article
• Authors: Veerapandian, Bhuvaneshwari; Shanmugam, Saravanan Ramiah; Varadhan, Srivathsan; Sarwareddy, Kartik Kumar; Mani, Krishna Priya; Ponnusami, V.
• Is Part Of: Carbohydrate polymers. Volume 227(2020)
• Publication Date: 2020-01-01
• Subjects: Chicken feathers -- Acid hydrolysis -- Chicken feather peptone -- Levan production; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2019.115361
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: Chicken feather peptone (CFP) from poultry waste was synthesized. CFP was utilized as a low cost nutrient source for microbial biopolymer production. Levan produced from CFP was found to be non-toxic and biocompatible in cell lines. CFP could be utilized as a low-cost nutrient source in fermentation industry. Abstract: Chicken feather peptone (CFP) derived from poultry waste is a rich source of essential minerals and amino acids. This, along with suitable carbon source, can be used as a low cost complex supplemental nutrient source for microbial fermentation. In the present work, CFP blended with sucrose was evaluated for the production of levan using Bacillus subtilis MTCC 441. Amount of CFP added to the medium significantly influenced levan production and it was found that at a concentration 2 g/L, maximum levan yield of 0.26 ± 0.04 g/g sucrose was obtained. The levan yield obtained with CFP as a low cost supplemental nutrient source was comparable with that obtained from commercial medium (0.31 ± 0.02 g/g sucrose). Levan produced using CFP was tested on primary cell lines at various concentrations (100–1000 μM) and found to be non-toxic and bio-compatible in nature. This indicates that CFP could be used as low cost nutrient source for levan production.