Xanthene Dyes and Green LED for the Inactivation of Foodborne Pathogens in Planktonic and Biofilm States. (16th April 2019)

• Record Type: Journal Article
• Authors: Silva, Alex Fiori; dos Santos, Adriele Rodrigues; Trevisan, Daliah Alves Coelho; Bonin, Edineia; Freitas, Camila Fabiano; Batista, Andreia Farias Pereira; Hioka, Noboru; Simões, Manuel; Graton Mikcha, Jane Martha
• Is Part Of: Photochemistry and photobiology. Volume 95:Number 5(2019)
• Page Start: 1230
• Page End: 1238
• Publication Date: 2019-04-16
• Subjects: erythrosine -- photodynamic inactivation -- rose bengal -- Salmonella Typhimurium -- Staphylococcus aureus; Photochemistry -- Periodicals; Light -- Physiological effect -- Periodicals; 541.35
• DOI: 10.1111/php.13104
• Languages: English
• ISSNs: 0031-8655

Abstract

Abstract: This study evaluated the rose bengal‐ and erythrosine‐mediated photoinactivation against Salmonella Typhimurium and Staphylococcus aureus planktonic and sessile cells using green LED as a light source. The free‐living or 2‐day‐old biofilm cells were treated with different concentrations of the photosensitizing agents and subjected to irradiation. Only 5 min photosensitization with rose bengal at 25 nmol L −1 and 75 μ mol L −1 completely eliminated S. aureus and S . Typhimurium planktonic cells, respectively. Erythrosine at 500 nmol L −1 and 5 min of light exposure also reduced S. aureus planktonic cells to undetectable levels. Eradication of S. aureus biofilms was achieved when 500 μ mol L −1 of erythrosine or 250 μ mol L −1 of rose bengal was combined with 30 min of irradiation. Scanning electron microscopy allowed the observation of morphological changes in planktonic cells and disruption of the biofilm architecture after photodynamic treatment. The overall data demonstrate that rose bengal and erythrosine activated by green LED may be a targeted strategy for controlling foodborne pathogens in both planktonic and sessile states. Abstract : Survival of S. aureus (A) biofilm and (B) planktonic cells photoinactivated with rose bengal.