Biochemical characterization and thermodynamic study of β-mannanase from Enterobacter asburiae. (July 2019)

• Record Type: Journal Article
• Authors: Dhiman, Shikha; Singh, Saumya; Singh, Gursharan; Khatri, Madhu; Arya, Shailendra Kumar
• Is Part Of: Biocatalysis and agricultural biotechnology. Number 20(2019)
• Publication Date: 2019-07
• Subjects: β-mannanase -- Enterobacter asburiae SD26 -- Kinetics -- Thermodynamics; Agricultural biotechnology -- Periodicals; Enzymes -- Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.bcab.2019.101211
• Languages: English
• ISSNs: 1878-8181

Abstract

Abstract: This study aimed for the partial purification, biochemical characterization, determination of bioprocess and thermodynamic parameters of mannanase from newly isolated strain Enterobacter asburiae SD26 under submerged culture. From different variables, locust bean gum and combination of peptone + yeast extract was found as best carbon and nitrogen sources respectively. The profiles of mannanases production and growth kinetics of E. asburiae SD26 were similar. The maximum specific growth rate (μmax ) and productivity ( γ Pmax ) of enzyme was 0.02 h -1 and 0.006 g l - 1 hr -1 . The highest mannanases activity was achieved at pH 6.0 and temperature 50°C. The kinetic parameters of mannanases was Km = 25 mg.ml -1, Vmax = 2500 μmol.min -1 .ml -1 and thermodynamic parameters of enzyme, Δ H d °, Δ G d °, Δ S d ° were 67.74 KJmol -1, 105.19 KJmol -1, − 115.89 J.mol -1 .K -1 respectively, recorded for mathematical description of the mannanase action at different process conditions. Highlights: A bacterial strain Enterobacter asburiae SD26 as mannanase producer was identified. Bioprocess parameters for growth of bacterial strain and mannanase production were estimated. Optimum pH and temperature conditions for high mannanase activity were determined. Kinetics and thermodynamics study of mannanase action were estimated.