Microvirga flavescens sp. nov., a novel bacterium isolated from forest soil and emended description of the genus Microvirga. Issue 3 (23rd January 2019)

• Record Type: Journal Article
• Authors: Zhang, Xian-jiao; Zhang, Jun; Yao, Qing; Feng, Guang-da; Zhu, Hong-Hui
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 3(2019)
• Page Start: 667
• Page End: 671
• Publication Date: 2019-01-23
• Subjects: forest soil -- Microvirga -- ANI; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003189
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : An aerobic, Gram-stain-negative, motile and rod-shaped bacterium, designated c27j1 T, was isolated from a forest soil sample from the Chebaling National Nature Reserve in Guangdong Province, China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain c27j1 T belongs to the genus Microvirga and was most closely related to Microvirga aerophila DSM 21344 T (97.7 %) and Microvirga subterranea DSM 14364 T (96.7 %). The average nucleotide identity and digital DNA–DNA hybridization values based on whole genome sequences of strain c27j1 T and M. aerophila DSM 21344 T were 77.2 and 22.4 %, respectively. It contained ubiquinone 10 as the predominant quinone, and C19 : 0 cyclo ω 8 c and summed feature 8 (C18 : 1 ω7 c and/or C18 : 1 ω6 c ) as the major fatty acids. The polar lipids consisted of phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine and two unidentified glycolipids. The genomic DNA G+C content based on the whole genome sequence was 62.2 mol%. Phenotypic, chemotaxonomic, phylogenetic and genomic analyses suggested that strain c27j1 T should represent a novel species of the genus Microvirga, for which the name Microvirga flavescens sp. nov. is proposed. The type strain is c27j1 T (=GDMCC 1.1356 T =KCTC 62433 T ). The description of the genus Microvirga is also emended, including the major fatty acids, genome size and DNA G+C content.