Chitinophaga silvisoli sp. nov., isolated from forest soil. Issue 4 (4th January 2019)

• Record Type: Journal Article
• Authors: Wang, Chunling; Lv, Yingying; Li, Anzhang; Feng, Guangda; Bao, Gegen; Zhu, Honghui; Tan, Zhiyuan
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 4(2019)
• Page Start: 909
• Page End: 913
• Publication Date: 2019-01-04
• Subjects: Bacteroidetes -- Chitinophaga -- phylogenetic tree; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003212
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, rod-shaped and aerobic bacterium, designated K20C18050901 T, was isolated from forest soil collected on 11 September 2017 from Dinghushan Biosphere Reserve, Guangdong Province, PR China (23° 10′ 24′′ N; 112° 32′ 10′′ E). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain K20C18050901 T belongs to the genus Chitinophaga, and showed the highest similarities to Chitinophaga sancti NBRC 15057 T (98.6 %) and Chitinophaga oryziterrae JCM 16595 T (96.9 %). The major fatty acids (>10 %) were iso-C15 : 0, C16 : 1 ω5 c, summed feature 3 (C16 : 1 ω6 c and/or C16 : 1 ω7 c ) and iso-C17 : 0 3-OH. The predominant respiratory quinone was menaquinone-7. The major polar lipid was phosphatidylethanolamine. The draft genome size of strain K20C18050901 T was 8.36 Mb with a DNA G+C content of 44.7 mol%. The digital DNA–DNA hybridization and average nucleotide identity values between strain K20C18050901 T and C. sancti NBRC 15057 T were 31.40 and 85.82 %, respectively. On the basis of phenotypic, genotypic and phylogenetic analysis, strain K20C18050901 T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga silvisoli sp. nov. is proposed. The type strain is K20C18050901 T (=GDMCC 1.1411 T =KCTC 62860 T ).