Effects of prophylactic administration of glutamine on CD4+ T cell polarisation and kidney injury in mice with polymicrobial sepsis. Issue 6 (11th June 2019)

• Record Type: Journal Article
• Authors: Hou, Yu-Chen; Wu, Jin-Ming; Chen, Kuen-Yuan; Chen, Po-Da; Lei, Cing-Syuan; Yeh, Sung-Ling; Lin, Ming-Tsan
• Is Part Of: British journal of nutrition. Volume 122:Issue 6(2019)
• Page Start: 657
• Page End: 665
• Publication Date: 2019-06-11
• Subjects: Glutamine, -- Sepsis, -- CD4+ T cells, -- T cell apoptosis, -- Kidney injury; Nutrition -- Periodicals; 572.4
• DOI: 10.1017/S0007114519000990
• Languages: English
• ISSNs: 0007-1145

Abstract

Abstract: The present study investigated the effects of glutamine (GLN) pretreatment on CD4 + T cell polarisation and remote kidney injury in mice with gut-derived polymicrobial sepsis. Mice were randomly assigned to three groups: normal control fed with American Institute of Nutrition (AIN)-93G diet and two sepsis groups provided with either AIN-93G-based diet or identical components, except part of casein was replaced by GLN. Mice were given their respective diets for 2 weeks. Then, mice in the sepsis groups were performed with caecal ligation and puncture and were killed 72 h after the surgery. Blood, spleens and kidneys were collected for further examination. The results showed that sepsis resulted in decreased circulating and splenic total T lymphocyte and CD4 + T cell percentages, whereas IL-4-, and forkhead box p3 (Foxp3)-expressing CD4 + T cells percentages were up-regulated. Compared with the sepsis control group, pretreatment with GLN maintained blood T and CD4 + T cells and reduced percentages of IL-4- and Foxp3-expressing CD4 + T cells. Also, a more pronounced activation and increased anti-apoptotic Bcl-2 gene expression of splenic CD4 + T cells were observed. Concomitant with the decreased plasma IL-6, keratinocyte-derived chemokine (KC) levels, the gene expression of KC, macrophage inflammatory protein-2 and renal injury biomarker kidney injury molecule-1 (Kim-1) were down-regulated when GLN was administered. These findings suggest that antecedent of GLN administration elicit a more balanced blood T helper cell polarisation, sustained T cell populations, prevented splenic CD4 + T cell apoptosis and attenuated kidney injury at late phase of polymicrobial sepsis. GLN may have benefits in subjects at risk of abdominal infection.