The effects of maternal and post-weaning diet interaction on glucose metabolism and gut microbiota in male mice offspring. Issue 3 (3rd June 2016)

• Record Type: Journal Article
• Authors: Zheng, Jia; Xiao, Xinhua; Zhang, Qian; Yu, Miao; Xu, Jianping; Qi, Cuijuan; Wang, Tong
• Is Part Of: Bioscience reports. Volume 36:Issue 3(2016)
• Publication Date: 2016-06-03
• Subjects: glucose homoeostasis -- gut microbiota -- high-fat diet -- maternal nutrition -- offspring; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1042/BSR20160103
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : Our data suggest that maternal and post-weaning high-fat (HF) diet predisposes the offspring for obesity, glucose intolerance and altered gut microbiota in later life. Our work is novel in showing the modulation of gut microbiota between maternal diet and glucose homoeostasis. Abstract : Substantial studies demonstrated that maternal nutrition can significantly determine the susceptibility to developing some metabolic diseases in offspring. However, investigations into the later-life effects of these diets on gut microbiota in the offspring are limited. Our objective was to explore the effects of maternal and post-weaning diet interaction on offspring's gut microbiota and glucose metabolism in later life. The male offspring of dams fed on either a high-fat (HF) diet or control (C) diet and then weaned to either a HF or C diet, generating four groups: C–C, HF–C, C–HF and HF–HF ( n =8 in each group). The C–C offspring had lower body weight than C–HF group at 16 weeks of age ( P <0.01) and both C–HF and HF–HF offspring had higher body weight than C–C group at 24 and 32 weeks of age ( P <0.001 respectively). The blood glucose (BG) levels of the male offspring from the C and HF dams weaned HF diet were significantly higher at 30 min, 60 min and 120 min ( P <0.001) after intraperitoneal glucose administration compared with those of the C–C group. The C–HF group had higher BG at 30 min than HF–HF group ( P <0.01). Furthermore, area under the curve (AUC) in C–HF and HF–HF groups was also significantly larger than C–C group ( P <0.001). Fasting BG and homoeostasis model assessment of insulin resistance (HOMA-IR) of the offspring were significantly higher in C–HF and HF–HF groups than C–C group at 32 weeks of age ( P <0.05). Operational taxonomic unit (OTU), Chao and Shannon indexes showed a significantly lower diversity in C–HF offspring compared with HF–C and C–C groups ( P <0.05). The dominant phyla of all the groups were Bacteroidetes, Firmicutes and Proteobacteria, which also showed significantly different percentages in the group ( P <0.05). Furthermore, it is indicated that Lactobacillus and Bacteroides were significantly associated with glucose response to a glucose load ( P <0.05). In conclusion, maternal and post-weaning diet interaction predisposes the offspring to aberrant glucose metabolism and alterations of gut microbiota in later life. Our study is novel in focusing on the effects of maternal and post-weaning diet interaction on offspring gut microbiota and glucose metabolism in later life.