All-polymer-based micro free-flow electrophoresis (μFFE) device with cellulose permeable membranes. (3rd September 2019)

• Record Type: Journal Article
• Authors: Takehara, Hiroaki; Sato, Shusuke; Ichiki, Takanori
• Is Part Of: Applied physics express. Volume 12:Number 10(2019)
• Publication Date: 2019-09-03
• Subjects: Microfluidics -- electrophoresis -- electroosmotic flow -- cellulose; Physics -- Periodicals; Technology -- Periodicals; 621.05
• DOI: 10.7567/1882-0786/ab3d64
• Languages: English
• ISSNs: 1882-0778

Abstract

Abstract: A micro free-flow electrophoresis ( μ FFE) device with cellulose membranes as ion-permeable separators was developed. Due to the neutral surface zeta potential of poly(methyl methacrylate-co-styrene), electroosmotic flow was not generated. The developed μ FFE device was evaluated in separations of two fluorescent dyes, rhodamine B and sulforhodamine B. Peak resolution ( Rs ) at an electric field strength of 11.5 V mm −1 was 4.1, 2.8, and 1.8 at sample flow velocities of 1.3, 2.0, and 2.7 mm s −1, respectively. All-polymer-based μ FFE devices are ready for use without pretreatment and satisfy the criteria for practical biological sample separation and purification in biomedical applications.