3D graphene-cellulose nanofiber hybrid scaffolds for cortical reconstruction in brain injuries. (5th September 2019)

• Record Type: Journal Article
• Authors: Hwang, Do Won; Park, Jong Bo; Sung, Dongchul; Park, Subeom; Min, Kyung-Ah; Kim, Kyu Wan; Choi, Yoori; Kim, Han Young; Lee, Eunji; Kim, Han Soo; Jin, Mo Ses; Park, Minsung; Song, Yoo Sung; Park, Jinwoo; Hyun, Jinho; Hong, Suklyun; Cho, Sung-Pyo; Hong, Byung Hee; Lee, Dong Soo
• Is Part Of: 2D materials. Volume 6:Number 4(2019)
• Publication Date: 2019-09-05
• Subjects: graphene oxide -- bacterial cellulose -- cortex-mimetic 3D hybrid scaffold -- corticectomized rat -- photoacoustic; Graphene -- Periodicals; Materials science -- Periodicals; Nanostructured materials -- Periodicals; 620.115
• DOI: 10.1088/2053-1583/ab3889
• Languages: English
• ISSNs: 2053-1583

Abstract

Abstract: Designing the nature-driven 3D scaffold is essential for reconstructing of the injured brain in association with stem cell replacement therapy. In this paper, we developed brain cortex-mimetic 3D hybrid scaffolds and applied them to a motor-cortectomy rat model. Graphene oxide bacterial cellulose (GO-BC) hybrid scaffold integrated GOs stably and homogeneously within BC nanofibrous building blocks made of BC and amphiphilic comb-like polymers (APCLP). Density functional theory calculations and molecular dynamics simulations revealed higher binding energies between GO-BC and APCLP than between GO or APLCP with BC. The monodispersed human neural stem cells (F3 cells) incorporated within the GO-BC scaffold generated a large number of differentiated neurons with robust neurite outgrowths and possible synapse formation in vitro . In corticectomized rats and nude mice, highly sensitive photoacoustic signals visualized the GO-BC at the implant site. Moreover, the implanted F3 cells within GO-BC were found to survive/proliferate and differentiate to neuronal lineage from the showing neuronal and synapse markers shown on ex vivo immunofluorescence staining in bioluminescence imaging. Cortex-mimetic and stem cell-instructive monodisperse GO-BC hybrid scaffolds are likely to be appropriate nanoplatforms for stem cell implantation to reconstruct injured/lost brain tissues and actively differentiate neural stem cells.