Trichloroethylene and its metabolite TaClo lead to degeneration of substantia nigra dopaminergic neurones: Effects in wild type and human A30P mutant α-synuclein mice. (15th October 2019)

• Record Type: Journal Article
• Authors: Keane, P.C.; Hanson, P.S.; Patterson, L.; Blain, P.G.; Hepplewhite, P.; Khundakar, A.A.; Judge, S.J.; Kahle, P.J.; LeBeau, F.E.N.; Morris, C.M.
• Is Part Of: Neuroscience letters. Volume 711(2019)
• Publication Date: 2019-10-15
• Subjects: PD Parkinson's disease -- TCE trichloroethylene -- TaClo 1-trichloromethyl-1, 2, 3, 4-tetrahydro-β-carboline -- DA dopaminergic -- SNpc substantia nigra pars compacta -- DMSO dimethylsulfoxide -- MPTP 1-methy, 4-phenyl, 1, 2, 3, 6 tetrahyrdopyridine -- CE coefficient of error -- TH tyrosine hydroxylase -- ANOVA analysis of variance; Trichloroethylene -- TaClo -- Parkinson's disease -- α-Synuclein -- Neurodegeneration; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2019.134437
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: Parkinson's disease (PD) may have an environmental component involving toxin exposure. Trichloroethylene (TCE) is a major environmental contaminant and can convert to the toxin TaClo. We administered TCE and TaClo to wild type and alpha-synuclein mutant mice as a model of PD. TCE and TaClo caused substantia nigra neurone loss but alpha-synuclein mutation was not additive. Abstract: Parkinson's disease (PD) is characterised pathologically by degeneration of the dopaminergic (DA) neurones of the substantia nigra pars compacta (SNpc) and the presence of α-synuclein containing Lewy body inclusions. Trichloroethylene (TCE) has been suggested as a potential environmental chemical that may contribute to the development of PD, via conversion to the neurotoxin, 1-Trichloromethyl-1, 2, 3, 4-tetrahydro-β-carboline (TaClo). We investigated the effect of an 8 week exposure to TCE or TaClo on wild type and, as an experimental model of PD, A30P mutant α-synuclein overexpressing mice using a combination of behaviour and pathology. TCE or TaClo exposure caused significant DA neuronal loss within the SNpc in both wild type and transgenic mice. Cell numbers were lower in A30P animals than wild type, however, no additive effect of TCE or TaClo exposure and A30P overexpression was found. TCE or TaClo did not appear to lead to acceleration of motor or cognitive deficits in either wild type or A30P mutant mice, potentially because of the modest reductions of DA neuronal number in the SNpc. Our results do however suggest that TCE exposure could be a possible factor in development of PD like changes following exposure.